Tetraploid development in the mouse
Article first published online: 27 AUG 2003
Copyright © 2003 Wiley-Liss, Inc.
Volume 228, Issue 4, pages 751–766, December 2003
How to Cite
Eakin, G. S. and Behringer, R. R. (2003), Tetraploid development in the mouse. Dev. Dyn., 228: 751–766. doi: 10.1002/dvdy.10363
- Issue published online: 18 NOV 2003
- Article first published online: 27 AUG 2003
- Manuscript Accepted: 19 JUN 2003
- Manuscript Received: 18 APR 2003
- U.S. Department of Defense Breast Cancer Research Program Predoctoral Fellowship
- genome duplication;
- nuclear transplantation;
- cell fusion;
- mouse chimeras
Spontaneous duplication of the mammalian genome occurs in approximately 1% of fertilizations. Although one or more whole genome duplications are believed to have influenced vertebrate evolution, polyploidy of contemporary mammals is generally incompatible with normal development and function of all but a few tissues. The production of tetraploid (4n) embryos has become a common experimental manipulation in the mouse. Although development of tetraploid mice has generally not been observed beyond midgestation, tetraploid:diploid (4n:2n) chimeras are widely used as a method for rescuing extraembryonic defects. The tolerance of tissues to polyploidy appears to be dependent on genetic background. Indeed, the recent discovery of a naturally tetraploid rodent species suggests that, in rare genetic backgrounds, mammalian genome duplications may be compatible with the development of viable and fertile adults. Thus, the range of developmental potentials of tetraploid embryos remains in large part unexplored. Here, we review the biological consequences and experimental utility of tetraploid mammals, in particular the mouse. Developmental Dynamics 228:751–766, 2003. © 2003 Wiley-Liss, Inc.