This article is a US Government work and, as such, is in the public domain in the United States of America.
Growth/differentiation factor 5 enhances chondrocyte maturation†
Article first published online: 25 AUG 2003
Published 2003 Wiley-Liss, Inc.
Volume 228, Issue 2, pages 208–216, October 2003
How to Cite
Coleman, C. M. and Tuan, R. S. (2003), Growth/differentiation factor 5 enhances chondrocyte maturation. Dev. Dyn., 228: 208–216. doi: 10.1002/dvdy.10369
- Issue published online: 16 SEP 2003
- Article first published online: 25 AUG 2003
- Manuscript Accepted: 20 JUN 2003
- Manuscript Received: 23 NOV 2002
- National Institutes of Health
- growth/differentiation factor 5;
- limb development;
- joint formation;
- chondrocyte hypertrophy
Growth/differentiation factor 5 (GDF5) is required for limb mesenchymal cell condensation and joint formation during skeletogenesis. Here, we use a model consisting of long-term, high-density cultures of chick embryonic limb mesenchymal cells, which undergo the entire life history of chondrocyte development, to examine the effects of GDF5 overexpression on chondrocyte maturation. Exposure to GDF5 significantly enhanced chondrocyte hypertrophy and maturation, as determined by the presence of alkaline phosphatase activity, collagen type X protein production, and the presence of a sulfated proteoglycan-rich extracellular matrix. Histologic analysis also revealed an increase in cell volume and cellular encasement in larger lacunae in GDF5-treated cultures. Taken together, these results support a role for GDF5 in influencing chondrocyte maturation and the induction of hypertrophy in the late stages of embryonic cartilage development, and provide additional mechanistic insights into the role of GDF5 in skeletal development. Development Dynamics 228:208–217, 2003. Published 2003 Wiley-Liss, Inc.