In ovo electroporation of avian somites
Article first published online: 9 FEB 2004
Copyright © 2004 Wiley-Liss, Inc.
Special Issue: Chick as a Model System
Volume 229, Issue 3, pages 643–650, March 2004
How to Cite
Scaal, M., Gros, J., Lesbros, C. and Marcelle, C. (2004), In ovo electroporation of avian somites. Dev. Dyn., 229: 643–650. doi: 10.1002/dvdy.10433
- Issue published online: 20 FEB 2004
- Article first published online: 9 FEB 2004
- Manuscript Accepted: 4 AUG 2003
- Manuscript Received: 28 FEB 2003
- European Community, MNERT. Grant Number: MCFI-2000-01593
- chick embryo
In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development. Developmental Dynamics 229:643–650, 2004. © 2004 Wiley-Liss, Inc.