In ovo electroporation of avian somites

Authors

  • Martin Scaal,

    1. Laboratoire de Génétique et de Physiologie du Développement (LGPD), Developmental Biology Institute of Marseille (IBDM), CNRS UMR 654, University Aix-Marseille II, Campus de Luminy, Marseille, France
    Current affiliation:
    1. Institut für Anatomie und Zellbiologie, Universität Freiburg, Albertstr. 17, 79104 Freiburg, Germany
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  • Jerome Gros,

    1. Laboratoire de Génétique et de Physiologie du Développement (LGPD), Developmental Biology Institute of Marseille (IBDM), CNRS UMR 654, University Aix-Marseille II, Campus de Luminy, Marseille, France
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  • Cynthia Lesbros,

    1. Laboratoire de Génétique et de Physiologie du Développement (LGPD), Developmental Biology Institute of Marseille (IBDM), CNRS UMR 654, University Aix-Marseille II, Campus de Luminy, Marseille, France
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  • Christophe Marcelle

    Corresponding author
    1. Laboratoire de Génétique et de Physiologie du Développement (LGPD), Developmental Biology Institute of Marseille (IBDM), CNRS UMR 654, University Aix-Marseille II, Campus de Luminy, Marseille, France
    • Laboratoire de Génétique et de Physiologie du Développement (LGPD), Developmental Biology Institute of Marseille (IBDM), CNRS UMR 654, University Aix-Marseille II, Campus de Luminy, Case 907, 13288 Marseille Cedex 09, France
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Abstract

In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development. Developmental Dynamics 229:643–650, 2004. © 2004 Wiley-Liss, Inc.

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