Time course of programmed cell death in Ciona intestinalis in relation to mitotic activity and MAPK signaling
Version of Record online: 22 APR 2004
Copyright © 2004 Wiley-Liss, Inc.
Volume 230, Issue 2, pages 251–262, June 2004
How to Cite
Tarallo, R. and Sordino, P. (2004), Time course of programmed cell death in Ciona intestinalis in relation to mitotic activity and MAPK signaling. Dev. Dyn., 230: 251–262. doi: 10.1002/dvdy.20055
- Issue online: 12 MAY 2004
- Version of Record online: 22 APR 2004
- Manuscript Received: 12 JAN 2004
- Manuscript Accepted: 12 JAN 2004
- EC. Grant Number: QLK3-CT2001-1890
- programmed cell death;
- Ciona intestinalis;
- MAPK signaling cascade;
- secondary neurogenesis
Programmed cell death (PCD) in the ascidian species Ciona intestinalis (Tunicata; Chordata) is investigated from early larvae to juvenile stages, by means of digoxigenin-based terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) technique. At first, PCD in the swimming larva affects trunk mesenchyme and central nervous system (CNS), then it participates extensively to metamorphosis, until it is restricted to developing organs of juveniles. Analysis of patterns of cell death and division in the larval CNS question old models on the genesis of the adult C. intestinalis brain. Upon performing immunochemical and functional assays for mitogen-activated protein kinase (MAPK) kinase kinase-1 (MEKK1), MAPK kinase 1/2 (MEK1/2), c-Jun NH2-terminal kinase (JNK), and dual phosphorylated extracellular regulated kinase 1/2 (dpERK1/2), the neurogenic competence of the larval brain appears to rely on a combinatorial regulation of PCD by the mitogen-activated protein kinase signaling cascade. These results show that, in tunicates, PCD consists of a multistep program implicated in growth and patterning with various roles. Developmental Dynamics 230:251–262, 2004. © 2004 Wiley-Liss, Inc.