Construction and analysis of a subtracted library and microarray of cDNAs expressed specifically in chicken heart progenitor cells

Authors

  • Mozhgan Afrakhte,

    1. Department of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts
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  • Thomas M. Schultheiss

    Corresponding author
    1. Department of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts
    • Division of Molecular and Vascular Medicine, RW-663, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215
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Abstract

A subtracted library was constructed of genes expressed specifically in the chick precardiac mesoendoderm. The subtracted library was obtained by hybridization of nucleic acids derived from a starting tester library of stage 4–7 chick precardiac mesoendoderm and a starting driver library of stage 2 area pellucida. Approximately 11,000 clones from the resulting subtracted library were printed onto a microarray. Screening of the microarray with probes derived from cardiac and noncardiac tissues, followed by in situ hybridization during chick embryo development, has identified multiple cardiac-specific genes, including several that have not been characterized previously. The microarray will be useful for future attempts to identify additional novel cardiac-specific genes, as well as to characterize patterns of gene expression during heart differentiation. Developmental Dynamics 230:290–298, 2004. © 2004 Wiley-Liss, Inc.

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