Early regeneration genes: Building a molecular profile for shared expression in cornea–lens transdifferentiation and hindlimb regeneration in Xenopus laevis
Article first published online: 4 JUN 2004
Copyright © 2004 Wiley-Liss, Inc.
Volume 230, Issue 4, pages 615–629, August 2004
How to Cite
Wolfe, A. D., Crimmins, G., Cameron, J. A. and Henry, J. J. (2004), Early regeneration genes: Building a molecular profile for shared expression in cornea–lens transdifferentiation and hindlimb regeneration in Xenopus laevis. Dev. Dyn., 230: 615–629. doi: 10.1002/dvdy.20089
- Issue published online: 8 JUL 2004
- Article first published online: 4 JUN 2004
- Manuscript Accepted: 16 FEB 2004
- Manuscript Received: 26 JAN 2004
- NIH/NEI. Grant Number: EY09844
- NIH. Grant Number: GM07283
- Xenopus laevis;
- limb regeneration;
- lens regeneration
Recent studies in Xenopus laevis have begun to compare gene expression during regeneration with that of the original development of specific structures (e.g., the hindlimb and lens), while other studies have sought differences in gene expression between regeneration-competent and regeneration-incompetent stages. To determine whether there are any similarities between the regeneration of different structures, we have used a differential screen to seek shared early gene expression between hindlimb regeneration and cornea–lens transdifferentiation in the Xenopus tadpole. We have isolated 13 clones representing genes whose expression is up-regulated within the first few days of both regenerating processes and which are not demonstrably up-regulated in the context of basic wound healing. Furthermore, all of these genes also show prominent late embryonic expression. The expression patterns and putative identities of all 13 genes are presented, and a model is considered that allows us to characterize and profile important changes in gene expression, which might be shared among various regenerating and developmental systems. Developmental Dynamics 230:615–629, 2004. © 2004 Wiley-Liss, Inc.