Patterns & Phenotypes
Expression patterns of claudin family of tight junction membrane proteins in developing mouse submandibular gland
Article first published online: 9 AUG 2004
Copyright © 2004 Wiley-Liss, Inc.
Volume 231, Issue 2, pages 425–431, October 2004
How to Cite
Hashizume, A., Ueno, T., Furuse, M., Tsukita, S., Nakanishi, Y. and Hieda, Y. (2004), Expression patterns of claudin family of tight junction membrane proteins in developing mouse submandibular gland. Dev. Dyn., 231: 425–431. doi: 10.1002/dvdy.20142
- Issue published online: 2 SEP 2004
- Article first published online: 9 AUG 2004
- Manuscript Accepted: 2 MAY 2004
- Manuscript Revised: 28 APR 2004
- Manuscript Received: 16 MAR 2004
- The Japan Science Society
- tight junction;
- submandibular gland
Here, we investigated the expression of the claudin family of tight junction transmembrane proteins in the developing mouse submandibular gland. Data obtained by reverse transcriptase-polymerase chain reaction, Western blot, and immunofluorescence microscopy showed the expression and localization of claudin-3 to -8, -10, and -11 at epithelial tight junctions. Examination of the glands taken from embryonic day (E) 14, E16, and newborn mice revealed differential expression patterns of these claudins in the developing epithelium. Claudin-3, -5, and -7 were expressed in all of the luminal epithelial cells of the ducts at all of the developmental stages examined and in those of terminal tubules at E16 and later. Claudin-4 was expressed mainly in the ducts at all the developmental stages. The expression of claudin-6 and -8 was also restricted to the ducts at E14 and E16; but after birth, the former was undetectable, whereas the latter was expressed in both the ducts and terminal tubules. Claudin-10 and -11 were detectable mainly in the terminal tubules at E16 and later. In addition to being found in the epithelium, claudin-5 was also expressed in certain mesenchymal cells, probably endothelial cells. These results will provide a valuable resource for further investigation of tubulogenesis and physiological regulation of claudin-based tight junctions. Developmental Dynamics 231:425–431, 2004. © 2004 Wiley-Liss, Inc.