Telomerase activity and differential expression of telomerase genes and c-myc in chicken cells in vitro
Version of Record online: 20 JUL 2004
Copyright © 2004 Wiley-Liss, Inc.
Volume 231, Issue 1, pages 14–21, September 2004
How to Cite
Swanberg, S. E., Payne, W. S., Hunt, H. D., Dodgson, J. B. and Delany, M. E. (2004), Telomerase activity and differential expression of telomerase genes and c-myc in chicken cells in vitro. Dev. Dyn., 231: 14–21. doi: 10.1002/dvdy.20149
- Issue online: 4 AUG 2004
- Version of Record online: 20 JUL 2004
- Manuscript Accepted: 1 JUN 2004
- Manuscript Revised: 31 MAY 2004
- Manuscript Received: 22 APR 2004
- USDA-NRI-CGP. Grant Number: 02-35205-11568
- Fredrick Cancer Research Facility (FCRF) of the National Cancer Institute, National Institutes of Health. Grant Number: 23XS002
- embryonic stem cells;
- chicken embryo fibroblasts;
This study examined telomerase activity and gene expression profiles for three genes in Gallus gallus domesticus: telomerase reverse transcriptase (chTERT), telomerase RNA (chTR), and c-myc. Expression of these genes was studied in chicken embryonic stem (chES) cells, chicken embryo fibroblasts (CEFs), and DT40 cells using quantitative real-time polymerase chain reaction. Our results establish that, relative to transcription levels in telomerase-negative CEFs, chTERT and chTR are up-regulated in telomerase-positive chES cells. Transcription levels of chTERT, chTR, and c-myc are dramatically up-regulated in telomerase-positive DT40 cells, relative to CEFs and chES cells. These results are consistent with a model in which telomerase activity is up-regulated in proliferating embryonic stem cells requiring stable telomeres to endure multiple rounds of cell division; down-regulated in differentiated, lifespan-limited cells; and dramatically up-regulated in immortalized, transformed cells for which uncontrolled proliferation is correlated with c-myc dysregulation and telomerase activity. Developmental Dynamics 231:14–21, 2004. © 2004 Wiley-Liss, Inc.