Patterns & Phenotypes

You have full text access to this OnlineOpen article

Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling

  1. Arndt F. Siekmann,
  2. Michael Brand*

Article first published online: 21 DEC 2004

DOI: 10.1002/dvdy.20248

Issue

Developmental Dynamics

Developmental Dynamics

Volume 232, Issue 2, pages 498–505, February 2005

Additional Information

How to Cite

Siekmann, A. F. and Brand, M. (2005), Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling. Dev. Dyn., 232: 498–505. doi: 10.1002/dvdy.20248

Author Information

  1. Max Planck Institute for Molecular Cell Biology and Genetics, and Department of Genetics, Dresden University of Technology, Dresden, Germany

*Department of Genetics, Dresden University of Technology, c/o Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany

Publication History

  1. Issue published online: 13 JAN 2005
  2. Article first published online: 21 DEC 2004
  3. Manuscript Revised: 17 SEP 2004
  4. Manuscript Accepted: 17 SEP 2004
  5. Manuscript Received: 25 AUG 2004

Funded by

  • Max-Planck-Society
  • International Max Planck Research School Dresden
  • European Union. Grant Number: QLG3-CT-2001-02310

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (1092K)

REFERENCES

  • Bellaiche Y, The I, Perrimon N. 1998. Tout-velu is a Drosophila homologue of the putative tumour suppressor EXT-1 and is needed for Hh diffusion. Nature 394: 85–88.
  • Blackhall FH, Merry CL, Davies EJ, Jayson GC. 2001. Heparan sulfate proteoglycans and cancer. Br J Cancer 85: 1094–1098.
  • Blagden CS, Currie PD, Ingham PW, Hughes SM. 1997. Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog. Genes Dev 11: 2163–2175.
  • Chen Y, Struhl G. 1996. Dual roles for patched in sequestering and transducing Hedgehog. Cell 87: 553–563.
  • Chen W, Burgess S, Hopkins N. 2001. Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity. Development 128: 2385–2396.
  • Clines GA, Ashley JA, Shah S, Lovett M. 1997. The structure of the human multiple exostoses 2 gene and characterization of homologs in mouse and Caenorhabditis elegans. Genome Res 7: 359–367.
  • Concordet JP, Lewis KE, Moore JW, Goodrich LV, Johnson RL, Scott MP, Ingham PW. 1996. Spatial regulation of a zebrafish patched homologue reflects the roles of sonic hedgehog and protein kinase A in neural tube and somite patterning. Development 122: 2835–2846.
  • Cook A, Raskind W, Blanton SH, Pauli RM, Gregg RG, Francomano CA, Puffenberger E, Conrad EU, Schmale G, Schellenberg G, et al. 1993. Genetic heterogeneity in families with hereditary multiple exostoses. Am J Hum Genet 53: 71–79.
  • Force A, Lynch M, Pickett FB, Amores A, Yan YL, Postlethwait J. 1999. Preservation of duplicate genes by complementary, degenerative mutations. Genetics 151: 1531–1545.
  • Han C, Lin X, Zhao C, Opoka R, Wang K, Lin X. 2001. Identification of botv, a new segment polarity gene that encodes a protein of the putative tumor suppressor EXT family. 42nd Annual Drosophila Research Conference. Washington, DC, Program No. 411.
  • Hecht JT, Hogue D, Strong LC, Hansen MF, Blanton SH, Wagner M. 1995. Hereditary multiple exostosis and chondrosarcoma: linkage to chromosome II and loss of heterozygosity for EXT-linked markers on chromosomes II and 8. Am J Hum Genet 56: 1125–1131.
  • Inatani M, Yamaguchi Y. 2003. Gene expression of EXT1 and EXT2 during mouse brain development. Brain Res Dev Brain Res 141: 129–136.
  • Katada T, Oogami S, Shima N, Kinoshita T. 2002. cDNA cloning and distribution of XEXT1, the Xenopus homologue of EXT1. Dev Genes Evol 212: 248–250.
  • Koziel L, Kunath M, Kelly OG, Vortkamp A. 2004. Ext1-dependent heparan sulfate regulates the range of Ihh signaling during endochondral ossification. Dev Cell 6: 801–813.
  • Kramer KL, Yost HJ. 2003. Heparan sulfate core proteins in cell-cell signaling. Annu Rev Genet 37: 461–484.
  • Krauss S, Concordet JP, Ingham PW. 1993. A functionally conserved homolog of the Drosophila segment polarity gene hh is expressed in tissues with polarizing activity in zebrafish embryos. Cell 75: 1431–1444.
  • Le Merrer M, Legeai-Mallet L, Jeannin PM, Horsthemke B, Schinzel A, Plauchu H, Toutain A, Achard F, Munnich A, Maroteaux P. 1994. A gene for hereditary multiple exostoses maps to chromosome 19p. Hum Mol Genet 3: 717–722.
  • Lin X, Wells D. 1997. Isolation of the mouse cDNA homologous to the human EXT1 gene responsible for hereditary multiple exostoses. DNA Seq 7: 199–202.
  • Lin X, Gan L, Klein WH, Wells D. 1998. Expression and functional analysis of mouse EXT1, a homolog of the human multiple exostoses type 1 gene. Biochem Biophys Res Commun 248: 738–743.
  • Lind T, Tufaro F, McCormick C, Lindahl U, Lidholt K. 1998. The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate. J Biol Chem 273: 26265–26268.
  • Nybakken K, Perrimon N. 2002. Heparan sulfate proteoglycan modulation of developmental signaling in Drosophila. Biochim Biophys Acta 1573: 280–291.
  • Reifers F, Bohli H, Walsh EC, Crossley PH, Stainier DY, Brand M. 1998. Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis. Development 125: 2381–2395.
  • Rubin JB, Choi Y, Segal RA. 2002. Cerebellar proteoglycans regulate sonic hedgehog responses during development. Development 129: 2223–2232.
  • Schauerte HE, van Eeden FJ, Fricke C, Odenthal J, Strahle U, Haffter P. 1998. Sonic hedgehog is not required for the induction of medial floor plate cells in the zebrafish. Development 125: 2983–2993.
  • Stickens D, Evans GA. 1997. Isolation and characterization of the murine homolog of the human EXT2 multiple exostoses gene. Biochem Mol Med 61: 16–21.
  • Stickens D, Brown D, Evans GA. 2000. EXT genes are differentially expressed in bone and cartilage during mouse embryogenesis. Dev Dyn 218: 452–464.
    Direct Link:
  • The I, Bellaiche Y, Perrimon N. 1999. Hedgehog movement is regulated through tout velu-dependent synthesis of a heparan sulfate proteoglycan. Mol Cell 4: 633–639.
  • Van Hul W, Wuyts W, Hendrickx J, Speleman F, Wauters J, De Boulle K, Van Roy N, Bossuyt P, Willems PJ. 1998. Identification of a third EXT-like gene (EXTL3) belonging to the EXT gene family. Genomics 47: 230–237.
  • Varga ZM, Amores A, Lewis KE, Yan YL, Postlethwait JH, Eisen JS, Westerfield M. 2001. Zebrafish smoothened functions in ventral neural tube specification and axon tract formation. Development 128: 3497–3509.
  • Wei G, Bai X, Gabb MM, Bame KJ, Koshy TI, Spear PG, Esko JD. 2000. Location of the glucuronosyltransferase domain in the heparan sulfate copolymerase EXT1 by analysis of Chinese hamster ovary cell mutants. J Biol Chem 275: 27733–27740.
  • Wise CA, Clines GA, Massa H, Trask BJ, Lovett M. 1997. Identification and localization of the gene for EXTL, a third member of the multiple exostoses gene family. Genome Res 7: 10–16.
  • Wuyts W, Van Hul W, Hendrickx J, Speleman F, Wauters J, De Boulle K, Van Roy N, Van Agtmael T, Bossuyt P, Willems PJ. 1997. Identification and characterization of a novel member of the EXT gene family, EXTL2. Eur J Hum Genet 5: 382–389.
  • Zak BM, Crawford BE, Esko JD. 2002. Hereditary multiple exostoses and heparan sulfate polymerization. Biochim Biophys Acta 1573: 346–355.
View Full Article (HTML) Get PDF (1092K)