Figure 3. The spatial expression patterns of XRTN2s and XRTN3.A: XRTN2-B. a: Stage (St.) 16, anterior side view. b: St. 19, dorsal side view, anterior to the left. c: St. 20, dissection view. d: Magnified view of c. The XRTN2-B transcript is localized in neural tissues, specifically the eye anlagen and neural plate (arrows). e: St. 21, dorsal view. f,g: St. 25, lateral view (f), dorsal view (g). h: St. 30, lateral view. i: St. 32, lateral view. XRTN2-B expression is restricted in the specific regions of the brain such as the prosencephalon, mesencephalon, and rhombencephalon. j: St. 40, lateral view. B: XRTN2-C. a: St. 17, dorsal side view, anterior to the left. b: St. 18, dorsal view. Arrowheads indicate the paraxial mesoderm. c: St. 19, dissection view. d: Magnified view of c. e,f: St. 20, anterior view (e), dorsal view (f). As the neural tube closes, XRTN2-C expression spreads posterior–lateral side, which is the posterior paraxial mesoderm. g,h: St. 22, lateral view (g), dorsal view (h). i: St. 24, dissection view. j: Magnified view of i. The XRTN2-C expression is restricted in myotome of somite. k: St 30, lateral view. l: St. 35, lateral view. The XRTN2-C expression is specified in the specific regions of the brain, the prosencephalon, mesencephalon, and rhombencephalon, as well as the myotome. m: St. 40 lateral view. C: XRTN3. a: Four-cell stage, dorsolateral side view. b: St. 6, dorsolateral view. c: St. 8, lateral view. d,e: St. 10.5, animal view (d), ventrolateral view (e). The arrow in e indicates the blastopore lip. The maternal XRTN3 transcript is detected in the animal hemisphere of the blastula and gastrula stage embryos. f,g: St. 18, dorsal view (f), dissection view (g). h: Magnified view of g. At the neurula stage, the XRTN3 expression commences in neural tissues, including the neural plate (arrow) and anterior head structure. i: St. 21, dorsal view. j,k: St. 22, lateral view (j), dissection view (k). l: Magnified view of k. m: St. 23, dorsal view. Specific expression in the optic nerve and spinal cord can be observed. n: St. 25, lateral view. As the mid–tail bud stage is passed over, the XRTN3 transcript is expressed in the somite and branchial arch. o: St. 30, lateral view. In addition to the optic nerve, the otic vesicle has XRTN3 expression. p: St. 35, lateral view. The XRTN3 expression is roughly maintained in the neural tissue, including the head. D: Sense. No positive signals were detected with sense probes of XRTNs. a: XRTN2-B sense in situ. b: XRTN2-C sense in situ. c: XRTN3 sense in situ. ba, branchial arch; ea, eye anlagen; ey, eye; mc, mesencephalon; my, myotome; nc, notochord; np, neural plate; nt, neural tube; on, optic nerve; ov, otic vesicle; pc, prosencephalon; pm, paraxial mesoderm; rc, rhombencephalon; sc, spinal cord; sm, somite.
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