Figure 1. Expression of Zac1 gene in whole mouse embryos at embryonic day (E) 12 (A–E, in toto), E12.5 (F–M, in sections), and E14 (N–U, in sections) by in situ hybridizations. A–E: At E12, intense expression is observed in telencephalic vesicles (tv in A), neural retina (nr in A), the vascular system of the hindbrain (black arrows in A), lateral dermomyotomes (white arrowheads in B), the ventral part of the somites and sclerotome (white arrows in B,C), the cartilage primordium of humerus (hmc in D), and the cartilage condensation of the digital zone of the forelimb (cc in E), as well as the vascular cells that surround the cartilaginous digits of the interdigital zone (open arrows in E). F–M: At E12.5, intense expression is detected in the inner (neural) layer of retina (nlr in F), extrinsic ocular muscle (eom in F), optic recess (or in F), neopallial cortex in the region of the future olfactory lobe (nc in F), ventricular zone of the telencephalic vesicles (vz in G), vascular cells of the interdigital zone (idiz, open arrows in H), and humeral (hmc in H,K and double asterisk in J), radial (rmc in H), phalangeal (double asterisk in H), metacarpal (mc in H) and carpal (single asterisk in H) mesenchymal condensations, as well as in the dorsal premuscle mesenchymal condensation (dmc in H, solid arrows in K and single asterisk in J), cartilage primordium of body vertebrae (cpbv in L), and mantle region of spinal cord in lumbosacral region (mrsc in M). In addition, moderate expression was observed in the neural tube (nt in I,K), in the notochord (n in K) and in the entrance of esophagus (eop in L). N–U: At E14, intense expression is observed in the neural tube (nt in N,P,Q,S,U), notochord (n in N,S), proliferating muscle cells (mpc in N,Q,R,S), muscle cells surrounding the ribs (mr in N), muscle cells that surround the femur (asterisk in N), radial nerve (rn in N), cartilage primordium of femur (cpf in N), cartilage primordium of ribs (cpr in N,P,U), cartilage primordium of neural arch (cpa in N), masseter muscle (mm in O), Meckel's cartilage (mc in O), cartilage primordium of acromion of left scapula (cpas in O), primordium of lower molar tooth (pmt in O), dorsal surface of tongue primordium (dst in O), tooth primordium (tp in O,T), primordium of shoulder joint (psj in Q and R), primordium of joint between tubercle of rib and neural arch of its own vertebra (pj in Q), muscle cells of the limb (mcl in R), cartilage primordium of humerus (cph in R), as well as in the hypothalamus (h in O,T), mamillothalamic tract (mta in O,T), ventricular zone of the telencephalic vesicles (vztv in O,T), neuroepithelium of the third ventricle (nIIIv in O,T), and the plexus choroids (pch in P). In addition, moderate expression is detected in the cartilage condensation primordium of sacral vertebral body (centrum, ccs in U) and aorta area (aa in N), whereas weak expression is observed in the esophagus (o in N), metanephros (definitive kidney, mn in S), and the muscle cells that surround the ribs (mcr in U).
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