This article was accepted for inclusion in Developmental Dynamics 233#2, June 2005–Special Focus on Limb Development.
Patterns & Phenotypes
Strategies to reduce variation in Xenopus regeneration studies†
Version of Record online: 19 JUL 2005
Copyright © 2005 Wiley-Liss, Inc.
Volume 234, Issue 1, pages 151–158, September 2005
How to Cite
Nye, H. L.D. and Cameron, J. A. (2005), Strategies to reduce variation in Xenopus regeneration studies. Dev. Dyn., 234: 151–158. doi: 10.1002/dvdy.20508
- Issue online: 16 AUG 2005
- Version of Record online: 19 JUL 2005
- Manuscript Accepted: 9 MAY 2005
- Manuscript Revised: 30 APR 2005
- Manuscript Received: 17 NOV 2004
- Xenopus laevis;
- statistical analysis;
- developmentally abnormal tadpoles
In this study, we present strategies for experimental design that minimize variation in Xenopus hindlimb regeneration results. We have standardized our laboratory culture conditions for older stage Xenopus tadpoles. We have established a normal tadpole growth curve for our laboratory and characterized normal tadpole behaviors in an effort to eliminate abnormal tadpoles from our experiments. We have used large sample sizes and statistical analysis to establish normal regeneration performances for seven amputation planes in stages 55–57 tadpole hindlimbs. We have demonstrated that regeneration performance of abnormal tadpoles is significantly different than that of normal tadpoles. We have examined the kinetics of ossification in developing Xenopus hindlimbs, and have found that increasing ossification rates and rates of regeneration decline are inversely correlated. Developmental Dynamics 234:151–158, 2005. © 2005 Wiley-Liss, Inc.