This article was accepted for inclusion in Developmental Dynamics 233#2, June 2005–Special Focus on Limb Development.
Transcriptome analysis of the murine forelimb and hindlimb autopod†
Article first published online: 29 JUL 2005
Copyright © 2005 Wiley-Liss, Inc.
Volume 234, Issue 1, pages 74–89, September 2005
How to Cite
Shou, S., Scott, V., Reed, C., Hitzemann, R. and Stadler, H. S. (2005), Transcriptome analysis of the murine forelimb and hindlimb autopod. Dev. Dyn., 234: 74–89. doi: 10.1002/dvdy.20514
- Issue published online: 16 AUG 2005
- Article first published online: 29 JUL 2005
- Manuscript Accepted: 8 JUN 2005
- Manuscript Revised: 22 APR 2005
- Manuscript Received: 3 JAN 2005
- Shriners Hospital for Children Research (HSS)
- National Institutes of Health (HSS)
- Biostatistics Shared Resource of the OHSU Cancer Institute. Grant Number: P30 CA69533
- distal limb development;
- gene expression;
To gain insight into the coordination of gene expression profiles during forelimb and hindlimb differentiation, a transcriptome analysis of mouse embryonic autopod tissues was performed using Affymetrix Murine Gene Chips (MOE-430). Forty-four transcripts with expression differences higher than 2-fold (T test, P ≤0.05) were detected between forelimb and hindlimb tissues including 38 new transcripts such as Rdh10,Frzb, Tbx18, and Hip that exhibit differential limb expression. A comparison of gene expression profiles in the forelimb, hindlimb, and brain revealed 24 limb-signature genes whose expression was significantly enriched in limb autopod versus brain tissue (fold change >2, P ≤ 0.05). Interestingly, the genes exhibiting enrichment in the developing autopod also segregated into significant fore- and hindlimb-specific clusters (P ≤ 0.05) suggesting that by E 12.5, unique gene combinations are being used during the differentiation of each autopod type. Developmental Dynamics 234:74–89, 2005. © 2005 Wiley-Liss, Inc.