Patterns & Phenotypes
Identification of a novel gene involved in asexual organogenesis in the budding ascidian Botryllus schlosseri
Article first published online: 28 SEP 2005
Copyright © 2005 Wiley-Liss, Inc.
Volume 234, Issue 4, pages 997–1005, December 2005
How to Cite
Laird, D. J., Chang, W.-T., Weissman, I. L. and Lauzon, R. J. (2005), Identification of a novel gene involved in asexual organogenesis in the budding ascidian Botryllus schlosseri. Dev. Dyn., 234: 997–1005. doi: 10.1002/dvdy.20583
- Issue published online: 16 NOV 2005
- Article first published online: 28 SEP 2005
- Manuscript Accepted: 10 AUG 2005
- Manuscript Revised: 18 JUL 2005
- Manuscript Received: 11 JUN 2005
- NIH. Grant Numbers: DK54762-02, 1R15GM067746-01
- Union College
- asexual budding;
Development via regeneration or budding shares some known genetic pathways with embryogenesis, but no concerted effort has been made to identify genes unique to asexual development. We have identified a novel gene that plays a role in cyclical bud formation and asexual organogenesis in the colonial ascidian Botryllus schlosseri. Athena mRNA is transcribed at high levels during the 24- to 36-hr interval of programmed cell death and new bud initiation at the conclusion of the budding cycle (takeover). Knockdown of Athena by RNAi and antisense morpholinos induced defects in the development of new buds ranging from retardation in growth and abnormal organogenesis to hollow buds lacking organs. As genetic intervention in this organism has not been possible, this study establishes the use of RNAi and morpholinos in Botryllus as well as describing the knockdown phenotype of a new gene. Developmental Dynamics 234:997–1005, 2005. © 2005 Wiley-Liss, Inc.