BMP and FGF-2 regulate neurogenin-2 expression and the differentiation of sensory neurons and glia
Article first published online: 19 JAN 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 235, Issue 3, pages 646–655, March 2006
How to Cite
Ota, M. and Ito, K. (2006), BMP and FGF-2 regulate neurogenin-2 expression and the differentiation of sensory neurons and glia. Dev. Dyn., 235: 646–655. doi: 10.1002/dvdy.20673
- Issue published online: 7 FEB 2006
- Article first published online: 19 JAN 2006
- Manuscript Accepted: 22 NOV 2005
- Sasakawa Scientific Research Grant from The Japan Science Society
- neural crest cells;
- sensory neurons;
- bone morphogenetic protein;
- fibroblast growth factor-2;
- notch signaling
We have examined the effects of signaling molecules and Notch signaling on the mechanisms regulating neurogenin (ngn) -2 expression. This ngn-2 is a transcription factor that is essential for the specification of early differentiating sensory neurons in the dorsal root ganglia. In the presence of bone morphogenetic protein (BMP), anti–ngn-2-positive cells appeared in mouse trunk neural crest cell cultures, and they expressed Brn3, indicating that ngn-2–expressing cells are sensory neurons. These cells did not differentiate after fibroblast growth factor (FGF) -2 treatment or after Notch activation. The suppression of ngn-2 expression by FGF-2 was recovered by treatment with a Notch signaling inhibitor. Thus, FGF-2 may prevent ngn-2 expression through Notch activation. Whereas BMP-4 inhibited glial differentiation, FGF-2 promoted gliogenesis by means of Notch activation. Our data suggest that BMP and FGF-2 act as positive and negative regulators in ngn-2 expression, respectively, and that these signaling molecules regulate the differentiation of sensory neurons and glia. Developmental Dynamics 235:646–655, 2006. © 2006 Wiley-Liss, Inc.