Neuronal leucine-rich repeat 6 (XlNLRR-6) is required for late lens and retina development in Xenopus laevis
Article first published online: 2 FEB 2006
Copyright © 2006 Wiley-Liss, Inc.
Special Issue: Campos-Ortega Special Focus
Volume 235, Issue 4, pages 1027–1041, April 2006
How to Cite
Wolfe, A. D. and Henry, J. J. (2006), Neuronal leucine-rich repeat 6 (XlNLRR-6) is required for late lens and retina development in Xenopus laevis. Dev. Dyn., 235: 1027–1041. doi: 10.1002/dvdy.20691
- Issue published online: 10 MAR 2006
- Article first published online: 2 FEB 2006
- Manuscript Accepted: 19 DEC 2005
- NIH/NEI. Grant Number: EY09844
- Summer Student Fellowship, Fight For Sight, Inc. Grant Number: SF05025
- Xenopus laevis;
- neuronal leucine rich repeat protein;
Leucine-rich repeat proteins expressed in the developing vertebrate nervous system comprise a complex, multifamily group, and little is known of their developmental function in vivo. We have identified a novel member of this group in Xenopus laevis, XlNLRR-6, and through sequence and phylogenetic analysis, have placed it within a defined family of vertebrate neuronal leucine-rich repeat proteins (NLRR). XlNLRR-6 is expressed in the developing nervous system and tissues of the eye beginning at the neural plate stage, and expression continues throughout embryonic and larval development. Using antisense morpholino oligonucleotide (MO) -mediated knockdown of XlNLRR-6, we demonstrate that this protein is critical for development of the lens, retina, and cornea. Reciprocal transplantation of presumptive lens ectoderm between MO-treated and untreated embryos demonstrate that XlNLRR-6 plays autonomous roles in the development of both the lens and retina. These findings represent the first in vivo functional analysis of an NLRR family protein and establish a role for this protein during late differentiation of tissues in the developing eye. Developmental Dynamics 235:1027–1041, 2006. © 2006 Wiley-Liss, Inc.