T. Langenberg and T. Dracz contributed equally to this work.
Analysis and visualization of cell movement in the developing zebrafish brain
Article first published online: 22 FEB 2006
Copyright © 2006 Wiley-Liss, Inc.
Special Issue: Campos-Ortega Special Focus
Volume 235, Issue 4, pages 928–933, April 2006
How to Cite
Langenberg, T., Dracz, T., Oates, A. C., Heisenberg, C.-P. and Brand, M. (2006), Analysis and visualization of cell movement in the developing zebrafish brain. Dev. Dyn., 235: 928–933. doi: 10.1002/dvdy.20692
- Issue published online: 10 MAR 2006
- Article first published online: 22 FEB 2006
- Manuscript Accepted: 19 DEC 2005
- DFG. Grant Number: SFB 655
- EU. Grant Number: QLG3-CT-2001-02310
- Max-Planck Society
- University of Technology Dresden
- midbrain–hindbrain boundary;
- isthmic organizer;
- lineage restriction;
Detailed reconstruction of the spatiotemporal history of embryonic cells is key to understanding tissue formation processes but is often complicated by the large number of cells involved, particularly so in vertebrates. Through a combination of high-resolution time-lapse lineage tracing and antibody staining, we have analyzed the movement of mesencephalic and metencephalic cell populations in the early zebrafish embryo. To facilitate the analysis of our cell tracking data, we have created TracePilot, a software tool that allows interactive manipulation and visualization of tracking data. We demonstrate its utility by showing novel visualizations of cell movement in the developing zebrafish brain. TracePilot (http://www.mpi-cbg.de/tracepilot) is Java-based, available free of charge, and has a program structure that allows the incorporation of additional analysis tools. Developmental Dynamics 235:928–933, 2006. © 2006 Wiley-Liss, Inc.