Expression of qBrn-1, a new member of the POU gene family, in the early developing nervous system and embryonic kidney

Authors

  • Lei Lan,

    1. The State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, Beijing, People's Republic of China
    2. Graduate School, the Chinese Academy of Sciences, Beijing, People's Republic of China
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  • Mingnan Liu,

    1. The State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, Beijing, People's Republic of China
    2. Graduate School, the Chinese Academy of Sciences, Beijing, People's Republic of China
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  • Yang Liu,

    1. The State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, Beijing, People's Republic of China
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  • Ying Liu,

    1. The State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, Beijing, People's Republic of China
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  • Wei Zhang,

    1. The State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, Beijing, People's Republic of China
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  • Jinxiao Xue,

    1. Biologie Moleculaire de la Differenciation, Universite Denis Diderot Paris 7, Case 7136, Paris, France
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  • Zhigang Xue,

    1. Biologie Moleculaire de la Differenciation, Universite Denis Diderot Paris 7, Case 7136, Paris, France
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  • Rongqiao He

    Corresponding author
    1. The State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, Beijing, People's Republic of China
    • The State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, the Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, PRC
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Abstract

It has been shown that POU domain genes play critical roles in the development of the nervous system. We have obtained a new member of the class III POU domain genes, qBrn-1, from the cDNA library of embryonic day 5 quail and have made an extensive expression pattern analysis of qBrn-1 and qBrn-2 throughout the early embryonic development by in situ hybridization. With a specific antibody we prepared, further analysis by immunohistochemistry showed that the location of qBrn-1 protein was consistent with that of the transcripts in the early developing quail. Our results showed that both qBrn-1 and qBrn-2 were preferentially expressed in the developing central nervous system, and their transcripts were initially detected in the neural plate and later in the distinct regions of the neural tube with a stage-dependent pattern. Moreover, their expression was also detected in both notochord and neural crests. However, qBrn-1 signal, different from qBrn-2, was more widely found in the auditory pits, branchial arches, and in the mesodermal components of the developing kidney. And the expression of qBrn-1 in nephric region was earlier and wider than that of mouse Brn-1, suggesting the characteristic function of qBrn-1 in the kidney formation. The distinct dynamic expression patterns of qBrn-1 and qBrn-2 indicate multiple roles of the class III POU genes in quail neurogenesis and organogenesis. Developmental Dynamics 235:1107–1114, 2006. © 2006 Wiley-Liss, Inc.

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