Transgenic Xenopus laevis strain expressing cre recombinase in muscle cells
Article first published online: 27 JUN 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 235, Issue 8, pages 2220–2228, August 2006
How to Cite
Waldner, C., Sakamaki, K., Ueno, N., Turan, G. and Ryffel, G. U. (2006), Transgenic Xenopus laevis strain expressing cre recombinase in muscle cells. Dev. Dyn., 235: 2220–2228. doi: 10.1002/dvdy.20880
- Issue published online: 17 JUL 2006
- Article first published online: 27 JUN 2006
- Manuscript Accepted: 23 MAY 2006
- Deutsche Forschungsgemeinschaft. Grant Number: RY 5/8-1
- Japan Society for the Promotion of Science. Grant Number: 17570176
- Cre recombinase;
- transgenic Xenopus laevis;
- conditional gene expression
For reproducible analyses of gene function in Xenopus, the use of transgenic strains is a promising approach but has limitations when investigating factors interfering with development. Therefore, inducible systems are attractive alternatives, and a binary system based on recombinases is a most versatile approach. We have shown previously that Cre and FLP recombinases are active in Xenopus laevis and can induce a silent reporter gene in a corresponding reporter strain. Here, we describe the establishment of the transgenic Xenopus laevis strain A7 expressing Cre recombinase under the control of the muscle-specific cardiac actin promoter. Upon crossing to several distinct reporter strains, A7 is able to induce EYFP, DsRed2, or LacZ reporter genes in a muscle-specific manner. This first Cre-expressing strain allows conditional activation of any gene of interest in muscle cells and, thus, opens up the use of recombinases as a new experimental strategy in Xenopus. Developmental Dynamics 235:2220–2228, 2006. © 2006 Wiley-Liss, Inc.