Drs. Nakamura and Nguyen contributed equally to this study.
Special Issue Patterns & Phenotypes
Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreERT to assay temporal activity windows along the proximodistal limb skeleton
Article first published online: 7 AUG 2006
Copyright © 2006 Wiley-Liss, Inc.
Special Issue: Mouse Development Special Issue
Volume 235, Issue 9, pages 2603–2612, September 2006
How to Cite
Nakamura, E., Nguyen, M.-T. and Mackem, S. (2006), Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreERT to assay temporal activity windows along the proximodistal limb skeleton. Dev. Dyn., 235: 2603–2612. doi: 10.1002/dvdy.20892
- Issue published online: 9 AUG 2006
- Article first published online: 7 AUG 2006
- Manuscript Accepted: 31 MAY 2006
- Center for Cancer Research, National Cancer Institute, NIH
- Tamoxifen-dependent Cre;
- Col2a1 transgenic promoter;
- chondrogenic deleter line;
- CreERT in cartilage
Cartilage differentiation occurs over a broad time range from early embryonic development, when the mesenchymal condensations that give rise to cartilage models for future bone first appear, and continuing through adult life, when there is ongoing maintenance of articular joint surfaces and re-activation of cartilage formation after fracture. The chondrogenic response also figures in the pathogenesis of degenerative and inflammatory joint diseases. We have generated a transgenic line expressing tamoxifen-dependent Cre recombinase that gives efficient recombination in the chondrogenic lineage, both during embryogenesis and postnatally, and provides a valuable tool for analysis of gene function selectively in chondrogenic cells using conditional genetic approaches. Because the cartilage model of the limb skeleton forms progressively in a proximodistal order during discrete, well-defined time periods, evaluation of the spatial extent of tamoxifen-induced recombination along the limb axis during these time windows has also enabled us to examine the pharmacokinetics of single-dose tamoxifen injections during pregnancy. Developmental Dynamics 235:2603–2612, 2006. © 2006 Wiley-Liss, Inc.