Global analysis of gene expression in Xenopus hindlimbs during stage-dependent complete and incomplete regeneration

Authors

  • Matthew Grow,

    Corresponding author
    1. Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana
    2. Center for Regenerative Biology and Medicine, Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana
    • Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 1345 W. 16th Street, Indianapolis, IN 46151
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  • Anton W. Neff,

    1. Center for Regenerative Biology and Medicine, Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana
    2. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Bloomington, Indiana
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  • Anthony L. Mescher,

    1. Center for Regenerative Biology and Medicine, Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana
    2. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Bloomington, Indiana
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  • Michael W. King

    1. Center for Regenerative Biology and Medicine, Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana
    2. Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Terre Haute, Indiana
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Abstract

Xenopus laevis tadpoles are capable of limb regeneration after amputation, in a process that initially involves the formation of a blastema. However, Xenopus has full regenerative capacity only through premetamorphic stages. We have used the Affymetrix Xenopus laevis Genome Genechip microarray to perform a large-scale screen of gene expression in the regeneration-complete, stage 53 (st53), and regeneration-incomplete, stage 57 (st57), hindlimbs at 1 and 5 days postamputation. Through an exhaustive reannotation of the Genechip and a variety of comparative bioinformatic analyses, we have identified genes that are differentially expressed between the regeneration-complete and -incomplete stages, detected the transcriptional changes associated with the regenerating blastema, and compared these results with those of other regeneration researchers. We focus particular attention on striking transcriptional activity observed in genes associated with patterning, stress response, and inflammation. Overall, this work provides the most comprehensive views yet of a regenerating limb and different transcriptional compositions of regeneration-competent and deficient tissues. Developmental Dynamics 235:2667–2685, 2006. © 2006 Wiley-Liss, Inc.

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