This article was accepted for inclusion in Developmental Dynamics 235 #9–Mouse Development Special Issue.
Special Issue Research Article
Expression and potential role of dentin phosphophoryn (DPP) in mouse embryonic tissues involved in epithelial–mesenchymal interactions and branching morphogenesis
Article first published online: 25 AUG 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 235, Issue 11, pages 2980–2990, November 2006
How to Cite
Alvares, K., Kanwar, Y. S. and Veis, A. (2006), Expression and potential role of dentin phosphophoryn (DPP) in mouse embryonic tissues involved in epithelial–mesenchymal interactions and branching morphogenesis. Dev. Dyn., 235: 2980–2990. doi: 10.1002/dvdy.20935
- Issue published online: 12 OCT 2006
- Article first published online: 25 AUG 2006
- Manuscript Accepted: 24 JUL 2006
- National Institute of Dental and Craniofacial Research. Grant Number: DE-01374
- National Institute of Diabetes and Digestive and Kidney Diseases. Grant Number: DK-28492
- dentin sialophosphoprotein;
- epithelial–mesenchymal interactions;
- kidney ureteric buds;
- lung epithelial alveolar buds
Dentin sialophosphoprotein (DSPP) is synthesized in both mesenchyme and epithelium at varying stages of tooth development. At the tooth cap stage, corresponding to embryonic day (E) 13.5 of mouse embryonic life, the phosphophoryn (DPP) portion of DSPP was immunohistochemically localized to the enamel organ with intense staining of oral ectoderm but no expression in dental follicle mesenchyme. Surprisingly, DPP was also expressed in ureteric bud branches of embryonic metanephric kidney and alveolar epithelial buds of developing lung. Reverse transcriptase-polymerase chain reaction analysis verified the presence of DSPP mRNA with identical sequences in the tooth, lung, and kidney. The DSPP−/− mouse with ablated DPP expression in the teeth, also exhibited aberrant organogenesis in kidney and lung. In the kidney, malformed metanephric S-shaped bodies and increased mesenchymal apoptosis were observed. Inclusion of anti-DPP antibodies in organ culture of metanephroi, harvested from E13.5 wild-type mice, likewise resulted in altered ureteric bud morphogenesis, suggesting a role for DPP in epithelial–mesenchymal interactions in meristic tissues during embryonic development. Developmental Dynamics 235:2980–2990, 2006. © 2006 Wiley-Liss, Inc.