Patterns & Phenotypes
Mes2, a MADF-containing transcription factor essential for Drosophila development
Article first published online: 6 OCT 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 235, Issue 12, pages 3387–3395, December 2006
How to Cite
Zimmermann, G., Furlong, E. E., Suyama, K. and Scott, M. P. (2006), Mes2, a MADF-containing transcription factor essential for Drosophila development. Dev. Dyn., 235: 3387–3395. doi: 10.1002/dvdy.20970
- Issue published online: 10 NOV 2006
- Article first published online: 6 OCT 2006
- Manuscript Accepted: 30 AUG 2006
- Ludwig Medical Research Foundation
The development of the Drosophila mesoderm is initiated by the basic helix-loop-helix transcription factor twist. We identified a gene encoding a putative transcription factor, mes2, in a screen for essential mesoderm-expressed genes that function downstream of twist. Mes2 protein belongs to a family of 48 Drosophila proteins containing MADF domains. MADF domains exist in worms, flies, and fish. Mes2 is a nuclear protein first produced in trunk and head mesoderm during late gastrulation. At later embryonic stages, mes2 is expressed in glia of the central and peripheral nervous systems, and in tissues derived from the head mesoderm. We have identified a null mutation of mes2 that leads to developmental arrest in first instar larvae. Increased production of Mes2 in multiple embryonic and larval tissues almost always causes lethality. The ubiquitous or epidermal misexpression of mes2 in the embryo causes a dramatic loss of epidermal integrity resulting in the failure of dorsal closure. Our data show that the precise regulation of mes2 expression is critical for normal development in Drosophila and implicate Mes2 in the regulation of essential target genes. Developmental Dynamics 235:3387–3395, 2006. © 2006 Wiley-Liss, Inc.