Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland
Version of Record online: 6 OCT 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 235, Issue 12, pages 3222–3229, December 2006
How to Cite
Ingman, W. V., Wyckoff, J., Gouon-Evans, V., Condeelis, J. and Pollard, J. W. (2006), Macrophages promote collagen fibrillogenesis around terminal end buds of the developing mammary gland. Dev. Dyn., 235: 3222–3229. doi: 10.1002/dvdy.20972
- Issue online: 10 NOV 2006
- Version of Record online: 6 OCT 2006
- Manuscript Accepted: 1 SEP 2006
- NIH. Grant Numbers: R01CA 94173, PO1 CA 100324
- Albert Einstein Cancer Center. Grant Number: P30 CA 13330
- mammary gland;
- multi-photon microscope;
Development of the ductal network in the mammary gland is dependent in part on the presence of macrophages. Here we utilize multi-photon microscopy and second harmonic generation to describe terminal end bud 3-dimensional structure and the organization of the surrounding collagen matrix. We have applied this approach to analyze the effect of macrophage deficiency on terminal end bud structure and collagen organization, using mice homozygous for a null mutation in the colony stimulating factor-1 gene (Csf1op/Csf1op). Primary terminal end buds have an oblong shape, with long collagen I fibers close to the neck of the terminal end bud and radiating upwards in the direction of growth. Around the terminal end buds, the amount of total collagen I detected by antibody staining was not affected by macrophage deficiency. However the amount of collagen I organized into long fibers, detected by second harmonic generation signal, was reduced in Csf1op/Csf1op mice. Macrophage deficiency also caused terminal end buds to be rounder and shorter. These studies reveal a role for macrophages in collagen fibrillogenesis and in organization of the structure of terminal end buds. Developmental Dynamics 235:3222–3229, 2006. © 2006 Wiley-Liss, Inc.