Eph–ephrin A system regulates murine blastocyst attachment and spreading
Article first published online: 12 OCT 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 235, Issue 12, pages 3250–3258, December 2006
How to Cite
Fujii, H., Tatsumi, K., Kosaka, K., Yoshioka, S., Fujiwara, H. and Fujii, S. (2006), Eph–ephrin A system regulates murine blastocyst attachment and spreading. Dev. Dyn., 235: 3250–3258. doi: 10.1002/dvdy.20977
- Issue published online: 10 NOV 2006
- Article first published online: 12 OCT 2006
- Manuscript Accepted: 4 SEP 2006
- Grants-in-Aid for Scientific Research. Grant Numbers: 16390474, 16659450
Although numerous adhesion molecules are expressed on mammalian endometrial epithelial cells, there have not been any studies of a mechanism to prevent premature attachment of the embryo. In this study, we examined the possible involvement of Eph–ephrin interaction, which can induce repulsive forces. In mice, Eph A1, A2, and A4 were expressed on endometrial epithelial cells and ephrin A1–4 on blastocysts. Reverse transcriptase-polymerase chain reaction showed that mRNA expression of ephrin A1–4 on embryos transiently decreased around the implantation period. Immunohistochemistry demonstrated that the expression of Eph A1 on endometrial epithelial cells and ephrin A1 and A3 expression on embryos decreased at implantation sites. Recombinant Eph A1 reacted with cell the surface of ephrin A-bearing trophectoderm cells. Attachment assays using Eph A1-coated dishes showed that blastocyst attachment was reversibly inhibited by Eph A1. These findings suggest an important role of the Eph–ephrin A system in regulating the initial embryo–maternal contact during the cross-talk period that precedes embryo implantation. Developmental Dynamics 235:3250–3258, 2006. © 2006 Wiley-Liss, Inc.