Targeted gene delivery to differentiated skeletal muscle: A tool to study dedifferentiation
Article first published online: 15 NOV 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 236, Issue 2, pages 481–488, February 2007
How to Cite
Morrison, J. I., Lööf, S., He, P., Aleström, P., Collas, P. and Simon, A. (2007), Targeted gene delivery to differentiated skeletal muscle: A tool to study dedifferentiation. Dev. Dyn., 236: 481–488. doi: 10.1002/dvdy.21019
- Issue published online: 10 JAN 2007
- Article first published online: 15 NOV 2006
- Manuscript Accepted: 18 OCT 2006
- Swedish Research Council. Grant Number: 20021937784641
- Swedish Foundation for Strategic Research
- Wenner-Gren Foundation
- Åke Wibergs Foundation
- Karolinska Institutet
- skeletal muscle;
Cellular dedifferentiation is required for functional regeneration in salamanders. Dedifferentiating multinucleate skeletal muscle gives rise to mononucleate cells during limb regeneration. Efficient methods and tools must be developed in order to understand the molecular cues underlying dedifferentiation. Here we describe a non-viral method to express extra-chromosomal DNA exclusively in terminally differentiated muscle without the need for cell purification steps. After cytoplasmic injection of various expression vectors into myotubes or myofibres, we detect long-lasting mRNA and protein expression in up to 70% of the injected cells. The combination of the transfection protocol with live imaging allows a time- and cost-effective screen of candidate genes in terminally differentiated muscle cells of both amphibian and mammalian origin. Developmental Dynamics 236:481–488, 2007. © 2006 Wiley-Liss, Inc.