Basolateral junctions are sufficient to suppress epithelial invasion during Drosophila oogenesis
Article first published online: 13 NOV 2006
Copyright © 2006 Wiley-Liss, Inc.
Volume 236, Issue 2, pages 364–373, February 2007
How to Cite
Szafranski, P. and Goode, S. (2007), Basolateral junctions are sufficient to suppress epithelial invasion during Drosophila oogenesis. Dev. Dyn., 236: 364–373. doi: 10.1002/dvdy.21020
- Issue published online: 10 JAN 2007
- Article first published online: 13 NOV 2006
- Manuscript Accepted: 12 OCT 2006
- NIH. Grant Number: RO1-CA87751
- Moran Foundation
- Mary Kay Ash Foundation
- basolateral junction;
- cell migration;
- tumor invasion;
Epithelial junctions play crucial roles during metazoan evolution and development by facilitating tissue formation, maintenance, and function. Little is known about the role of distinct types of junctions in controlling epithelial transformations leading to invasion of neighboring tissues. Discovering the key junction complexes that control these processes and how they function may also provide mechanistic insight into carcinoma cell invasion. Here, using the Drosophila ovary as a model, we show that four proteins of the basolateral junction (BLJ), Fasciclin-2, Neuroglian, Discs-large, and Lethal-giant-larvae, but not proteins of other epithelial junctions, directly suppress epithelial tumorigenesis and invasion. Remarkably, the expression pattern of Fasciclin-2 predicts which cells will invade. We compared the apicobasal polarity of BLJ tumor cells to border cells (BCs), an epithelium-derived cluster that normally migrates during mid-oogenesis. Both tumor cells and BCs differentiate a lateralized membrane pattern that is necessary but not sufficient for invasion. Independent of lateralization, derepression of motility pathways is also necessary, as indicated by a strong linear correlation between faster BC migration and an increased incidence of tumor invasion. However, without membrane lateralization, derepression of motility pathways is also not sufficient for invasion. Our results demonstrate that spatiotemporal patterns of basolateral junction activity directly suppress epithelial invasion by organizing the cooperative activity of distinct polarity and motility pathways. Developmental Dynamics 236:364–373, 2007. © 2006 Wiley-Liss, Inc.