Detection of mammalian microRNA expression by in situ hybridization with RNA oligonucleotides

Authors

  • Monika Deo,

    1. Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan
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  • Jenn-Yah Yu,

    1. Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan
    2. Program in Neuroscience, University of Michigan, Ann Arbor, Michigan
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  • Kwan-Ho Chung,

    1. Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan
    2. Program in Neuroscience, University of Michigan, Ann Arbor, Michigan
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  • Melissa Tippens,

    1. Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan
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  • David L. Turner

    Corresponding author
    1. Molecular and Behavioral Neuroscience Institute, University of Michigan, Ann Arbor, Michigan
    2. Program in Neuroscience, University of Michigan, Ann Arbor, Michigan
    3. Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan
    • Molecular and Behavioral Neuroscience Institute, University of Michigan, 5062 BSRB, 109 Zina Pitcher Place, Ann Arbor, MI 48109-2200
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In the original published version of this article, an erroneous concentration was published in the Experimental Procedures section. The original article included “100 mg/ml heparin” to describe the hybridization buffer, but it should correctly read “100 μg/ml heparin.” The corrected sentence is published in full below. The authors regret this error.

“Hybridization buffer: 50% formamide, 0.3 mg/ml yeast RNA (ICN), 100 μg/ml heparin, 1× Denhardt's solution (0.2% BSA, 0.2% polyvinylpyrrolidone, 0.2% Ficoll 400), 0.1% Tween 20, 0.1% CHAPS, 5 mM EDTA, and 0.3 nmol/ml 12-mer random sequence DNA oligonucleotides.”

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