A hypermorphic mouse Gli3 allele results in a polydactylous limb phenotype
Article first published online: 31 JAN 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 3, pages 769–776, March 2007
How to Cite
Wang, C., Pan, Y. and Wang, B. (2007), A hypermorphic mouse Gli3 allele results in a polydactylous limb phenotype. Dev. Dyn., 236: 769–776. doi: 10.1002/dvdy.21082
- Issue published online: 22 FEB 2007
- Article first published online: 31 JAN 2007
- Manuscript Accepted: 7 JAN 2007
- March of Dimes Foundation
- National Institutes of Health. Grant Number: R01CA111673
- Sonic hedgehog;
- limb patterning
Gli3 protein processing to generate the Gli3 repressor is mediated by proteasome and inhibited by Hedgehog signaling. The Gli3 repressor concentration is graded along the anterior–posterior axis of the developing vertebrate limb due to posteriorly restricted Sonic hedgehog expression. In this study, we created a small deletion at the Gli3 locus (Gli3Δ68), which causes a half reduction in the Gli3 repressor levels and a slightly increased activity of full-length mutant protein in the limb. Mice homozygous for Gli3Δ68 develop one to two extra partial digits in the anterior of the limb, while mice carrying one copy of the Gli3Δ68 allele die soon after birth and display seven digits. These phenotypes are more severe than those found in mice lacking one wild-type Gli3 allele. The expression of dHand, Hoxd12, and Hoxd13 is anteriorly expanded in the limb, even though no up-regulation of Gli1 and Ptc RNA expression is detected. These findings suggest that a decrease in the Gli3 repressor level in combination with an increase in Gli3 full-length activity results in more severe digit patterning abnormalities than those caused by a loss of one wild-type Gli3 allele. Developmental Dynamics 236:769–776, 2007. © 2007 Wiley-Liss, Inc.