Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems
Version of Record online: 5 FEB 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 3, pages 706–718, March 2007
How to Cite
Sassa, T., Aizawa, H. and Okamoto, H. (2007), Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems. Dev. Dyn., 236: 706–718. doi: 10.1002/dvdy.21084
- Issue online: 22 FEB 2007
- Version of Record online: 5 FEB 2007
- Manuscript Accepted: 24 DEC 2006
- Ministry of Education, Science, Technology, Sports, and Culture of Japan
- Japan Science and Technology Corporation
- The RIKEN Brain Science Institute
- octavolateral nucleus;
- torus semicircularis;
- lateral line
The dorsal hindbrain includes distinct classes of neurons for processing various sensory stimuli, but the developmental aspects of these neurons remain largely unknown. We identify here two distinct classes of neurons in the dorsal hindbrain of developing zebrafish: (1) neurons that express the inhibitory neuronal marker Gad1/2, and (2) neurons that express the zn-5 antigen and Lhx2/9 and require the basic helix–loop–helix transcription factor Atoh1a for development. Neurons were traced to their axon terminals by expressing green fluorescent protein using the Gal4VP16-UAS (UAS, upstream activating sequences) system in combination with the promoter/enhancer regions of gad2 for the Gad1/2(+) neurons and zic1 for the zn-5(+)Lhx2/9(+) neurons. The Gad1/2(+) neurons projected to the contralateral hindbrain, while the zn-5(+)Lhx2/9(+) neurons projected to the contralateral midbrain torus semicircularis, suggesting a role in auditory and lateral line sensory processing. Comparison of these projections with those from the cochlear nuclei to the inferior colliculus in mammals suggests similarities across vertebrate species. Developmental Dynamics 236:706–718, 2007. © 2007 Wiley-Liss, Inc.