Patterns & Phenotypes
Isoform-specific interaction of Flamingo/Starry Night with excess Bazooka affects planar cell polarity in the Drosophila wing
Article first published online: 15 FEB 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 4, pages 1064–1071, April 2007
How to Cite
Wasserscheid, I., Thomas, U. and Knust, E. (2007), Isoform-specific interaction of Flamingo/Starry Night with excess Bazooka affects planar cell polarity in the Drosophila wing. Dev. Dyn., 236: 1064–1071. doi: 10.1002/dvdy.21089
- Issue published online: 15 MAR 2007
- Article first published online: 15 FEB 2007
- Manuscript Accepted: 12 JAN 2007
- Deutsche Forschungsgemeinschaft. Grant Number: Kn250/15-3
- cell polarity;
- PDZ domain;
Epithelia display two types of polarity, apical–basal and planar cell polarity (PCP), and both are crucial for morphogenesis and organogenesis. PCP signaling pathways comprise transmembrane proteins, such as Flamingo/Starry Night, and cytoplasmic, membrane-associated proteins such as Dishevelled. During establishment of PCP in the Drosophila wing, PCP proteins accumulate apically in distinct “cortical domains” on proximal and distal plasma membranes. This finding suggests that their localized function depends on prior definition of apicobasal polarity. Here, we show that overexpression of Bazooka, a PDZ-domain protein essential for apicobasal polarity in the embryo, perturbs development of PCP, but has no effect on apicobasal polarity. The PCP phenotype is associated with a failure to restrict Flamingo/Starry night to the proximal and distal plasma membranes of the wing epithelium. We further demonstrate that flamingo expresses two differentially spliced RNAs in wing imaginal discs, which encode two isoforms of the atypical cadherin Flamingo. The predominant Starry night–type form contains a PDZ-binding motif, which mediates binding to Bazooka in vitro. Pull-down assays support the occurrence of such an interaction in wing imaginal discs. The results suggest that interaction between the apicobasal and planar cell polarity systems has to be tightly coordinated to ensure proper morphogenesis of the wing disc epithelium. Developmental Dynamics 236:1064–1071, 2007. © 2007 Wiley-Liss, Inc.