System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate

Authors

  • Deborah U. Frank,

    1. Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah
    Search for more papers by this author
  • Sarah A. Elliott,

    1. Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah
    Search for more papers by this author
  • Eon Joo Park,

    1. Department Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah
    Search for more papers by this author
  • Jennetta Hammond,

    1. Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah
    Search for more papers by this author
  • Yukio Saijoh,

    1. Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah
    2. Department Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah
    Search for more papers by this author
  • Anne M. Moon

    Corresponding author
    1. Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah
    2. Program in Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah
    3. Department Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah
    • Eccles Institute of Human Genetics, Room 4160B, 15 North 2030 East, Salt Lake City, UT 84112
    Search for more papers by this author

Abstract

We targeted the reverse tetracycline controlled transactivator (rtTA) to the Foxa2 locus (Foxa2ITA) to generate a system for regulating Cre-recombinase activity within Foxa2 expression domains, including the endoderm, notochord, and floor plate of early mouse embryos. The use of an internal ribosomal entry site to obtain rtTA expression preserves Foxa2 function of the targeted allele. Cre activity with this system reflects the level of endogenous Foxa2 activity and is also tightly controlled by doxycycline. The location of Cre activity within the broader Foxa2 expression domain can be restricted by altering the timing of doxycycline administration. Isolated floor plate expression can be obtained in this manner. This system will provide a useful tool for manipulating gene expression in endoderm, notochord, and floor plate, all of which are tissues with important structural and patterning functions during embryogenesis. Developmental Dynamics 236:1085–1092, 2007. © 2007 Wiley-Liss, Inc.

Ancillary