Patterns & Phenotypes
System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate
Article first published online: 15 FEB 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 4, pages 1085–1092, April 2007
How to Cite
Frank, D. U., Elliott, S. A., Park, E. J., Hammond, J., Saijoh, Y. and Moon, A. M. (2007), System for inducible expression of cre-recombinase from the Foxa2 locus in endoderm, notochord, and floor plate. Dev. Dyn., 236: 1085–1092. doi: 10.1002/dvdy.21093
- Issue published online: 15 MAR 2007
- Article first published online: 15 FEB 2007
- Manuscript Accepted: 16 JAN 2007
- NIH. Grant Number: RO1HD044157-01
- Primary Children's Medical Center Foundation
- Children's Health Research Center at the University of Utah
- floor plate;
We targeted the reverse tetracycline controlled transactivator (rtTA) to the Foxa2 locus (Foxa2ITA) to generate a system for regulating Cre-recombinase activity within Foxa2 expression domains, including the endoderm, notochord, and floor plate of early mouse embryos. The use of an internal ribosomal entry site to obtain rtTA expression preserves Foxa2 function of the targeted allele. Cre activity with this system reflects the level of endogenous Foxa2 activity and is also tightly controlled by doxycycline. The location of Cre activity within the broader Foxa2 expression domain can be restricted by altering the timing of doxycycline administration. Isolated floor plate expression can be obtained in this manner. This system will provide a useful tool for manipulating gene expression in endoderm, notochord, and floor plate, all of which are tissues with important structural and patterning functions during embryogenesis. Developmental Dynamics 236:1085–1092, 2007. © 2007 Wiley-Liss, Inc.