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The Supplementary Material referred to in this article can be viewed at http://www.interscience.wiley.com/jpages/1058-8388/suppmat

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jws-dvdy.21100.fig1.tif2047K Supplemental Figure 1. Tissue-specific damage to the heart upon exposure ofTg(cmlc2:CFP-NTR) s890 larvae to Mtz.Merged images and single channels of heart sections ofTg(cmlc2:CFP-NTR) s890 ; Tg(cmlc2:GFP)larvae treated with DMSO- (A-A???- magnification of Fig. 2E) and Mtz- (B-B??? - same as Fig. 2G) immunostained for activated Caspase-3 in blue (A?, B?) and stained with rhodamine phalloidin in red (A??, B??),Tg(cmlc2:GFP)in green (A???, B???). Activated Caspase-3 expression (B?) was detected exclusively inTg(cmlc2:GFP)expressing cells (B???) indicating that cell death occured specifically in cardiomyocytes and without affecting the neighboring CFP-NTR- cells.
jws-dvdy.21100.anim1A.mov6986K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movie A(t1).Bright field movie of controlTg(cmlc2:GFP)larva A exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, showing wild-type blood circulation.
jws-dvdy.21100.anim2A.mov5871K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies A(t2).Bright field movie of controlTg(cmlc2:GFP)larva A first exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, and then incubated in Mtz-free medium for 72 hours, showing wild-type blood circulation.
jws-dvdy.21100.anim3A.mov476K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movie A?(t1).Fluorescence movie (GFP expression) of controlTg(cmlc2:GFP)larva A exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, showing the wild-type contracting heart.
jws-dvdy.21100.anim4A.mov647K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies A?(t2).Fluorescence movie (GFP expression) of controlTg(cmlc2:GFP)larva A first exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, and then incubated in Mtz-free medium for 72 hours, showing the wild-type contracting heart.
jws-dvdy.21100.anim1B.mov5204K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B(t1) and C(t1).Bright field movies of Tg(cmlc2:CFP-NTR)s890;Tg(cmlc2:GFP)larvae, B and C, exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, showing failure in blood circulation, due to the heart?s inability to pump blood.
jws-dvdy.21100.anim2B.mov4790K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B(t2) and C(t2).Bright field movies ofTg(cmlc2:CFP-NTR)s890; Tg(cmlc2:GFP)larvae, B and C, exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours (t1), left to recover in Mtz-free medium for 72 hours. After 72 hours in Mtz-free medium these larvae have recovered blood circulation.
jws-dvdy.21100.anim3B.mov419K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B?(t1) and C?(t1).Fluorescence movies (GFP expression) ofTg(cmlc2:CFP-NTR)s890; Tg(cmlc2:GFP)larvae, B and C, exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, showing drastically reduced contractility of the collapsed atrium and ventricle.Regeneration of functional myocardium after severe cardiomyocyte damage.
jws-dvdy.21100.anim4B.mov625K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B?(t2) and C?(t2).Fluorescence movies (GFP expression) ofTg(cmlc2:CFP-NTR)s890; Tg(cmlc2:GFP)larvae, B and C, first exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, left to recover in Mtz-free medium for 72 hours. After 72 hours in Mtz-free medium the myocardial tissue of these larvae, previously damaged and unable to contract (t1), has recovered its morphology and contractility.
jws-dvdy.21100.anim1D.mov5741K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B(t1) and C(t1).Bright field movies of Tg(cmlc2:CFP-NTR)s890;Tg(cmlc2:GFP)larvae, B and C, exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, showing failure in blood circulation, due to the heart?s inability to pump blood.
jws-dvdy.21100.anim2C.mov4886K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B(t2) and C(t2).Bright field movies ofTg(cmlc2:CFP-NTR)s890; Tg(cmlc2:GFP)larvae, B and C, exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours (t1), left to recover in Mtz-free medium for 72 hours. After 72 hours in Mtz-free medium these larvae have recovered blood circulation.
jws-dvdy.21100.anim3C.mov440K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B?(t1) and C?(t1).Fluorescence movies (GFP expression) ofTg(cmlc2:CFP-NTR)s890; Tg(cmlc2:GFP)larvae, B and C, exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, showing drastically reduced contractility of the collapsed atrium and ventricle.Regeneration of functional myocardium after severe cardiomyocyte damage.
jws-dvdy.21100.anim4C.mov774K Conditional ablation of cardiomyocytes results in severe cardiac morphological and functional phenotypes. Movies B?(t2) and C?(t2).Fluorescence movies (GFP expression) ofTg(cmlc2:CFP-NTR)s890; Tg(cmlc2:GFP)larvae, B and C, first exposed to 10 mM Mtz (in 0.2% DMSO) at 48 hpf for 24 hours, left to recover in Mtz-free medium for 72 hours. After 72 hours in Mtz-free medium the myocardial tissue of these larvae, previously damaged and unable to contract (t1), has recovered its morphology and contractility.

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