Special Issue Reviews–A Peer Reviewed Forum
Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis
Article first published online: 6 MAR 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Ciona
Volume 236, Issue 7, pages 1758–1767, July 2007
How to Cite
Sasakura, Y. (2007), Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis. Dev. Dyn., 236: 1758–1767. doi: 10.1002/dvdy.21111
- Issue published online: 18 JUN 2007
- Article first published online: 6 MAR 2007
- Manuscript Accepted: 5 FEB 2007
- transgenic techniques;
- enhancer trap;
- insertional mutagenesis
Stable transgenesis is a splendid technique that is applicable to the creation of useful marker lines, enhancer/gene traps, and insertional mutagenesis. Recently, transposon-mediated transformation using a Tc1/mariner transposable element Minos has been reported in two ascidians: Ciona intestinalis and C. savignyi. The transposon derived from an insect, Drosophila hydei, has high activity for excision in Ciona embryos and transposition in their genome. As much as 37% of Minos-injected C. intestinalis transmitted transposon insertions to the subsequent generation. Minos-mediated germline transgenesis has also been achieved by means of electroporation method. Minos techniques have been applied to enhancer traps and insertional mutagenesis in Ciona. For those reasons, Minos offers the high potential for use as a powerful tool for future genetic studies. This review specifically addresses recent achievements of transformation techniques in Ciona, as exemplified using the Minos system. Developmental Dynamics 236:1758–1767, 2007. © 2007 Wiley-Liss, Inc.