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The Supplementary Material referred to in this article can be viewed at www.interscience.wiley.com/jpages/1058-8388/suppmat .

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jws-dvdy.21126.fig1.tif9376K Suppl. Fig. 1.Statistical analysis of differences in the zonal distribution of EGFP+ electroporated cells at E18.5. Statistical analysis of the zonal distribution of GFP+ cells electroporated at E12.5, E13.5, E14.5 and E15.5 and analyzed at E18.5 (from Fig. 2). Statistical significance was calculated in the GZ, IZ, IG and SG using Tukey?s post-hoc test for multiple comparisons using Graphpad Prizm Software (Graphpad Inc.). GZ, germinal zone; IG, infragranular; IZ, intermediate zone; SG, supragranular.
jws-dvdy.21126.fig2.tif13609K Suppl. Fig. 2.Comparing the position of proliferating S-phase progenitors and E15.5 electroporated cells in E18.5 embryos. Low (A-C) and high (D-F) magnification images of E18.5 embryos that were electroporated with a pCIG2-GFP construct at E15.5 (green cells, A, B, D, E) and administered BrdU 30 minutes prior to harvesting at E18.5 (red cells, A, C, D, F).
jws-dvdy.21126.fig3.tif9059K Suppl. Fig. 3.Statistical analysis of differences in the zonal distribution of EGFP+ electroporated cells at P7. Statistical analysis of the zonal distribution of GFP+ cells electroporated at E12.5, E13.5, E14.5 and E15.5 and analyzed at P7 (from Fig. 3). Statistical significance was calculated in layers II/III, IV, V and VI using Tukey?s post-hoc test for multiple comparisons using Graphpad Prizm Software (Graphpad Inc.).
jws-dvdy.21126.fig4.tif20546K Suppl. Fig. 4.Functional analysis of zebrafish 1.0kb ER81 regulatory element in the murine neocortex. (A) Schematic representation of zebrafish (z)ER81 _1.0kb::GFP reporter construct, which was co-electroporated with pCIG2-dsRed into the murine telencephalon at E14.5 and harvested at E18.5. (B-D) Visualization of E14.5 electroporated cells in neocortex at E18.5, with dsRed+ cells delineating the area of electroporation (red, B, C) and the absence of GFP+ cells showing no neocortical activity of the reporter. (E-G) Visualization of E14.5 electroporated cells in cingulate cortex at E18.5, with dsRed+ cells delineating the area of electroporation (red, E, F) and no GFP+ cells showing an absence of activity of pallial regulatory elements in the z ER81 _1.0kb reporter (green, E, G). (H-J) Visualization of E14.5 electroporated cells in thalamus at E18.5, with dsRed+ cells delineating the area of electroporation (red, H, I) and absence of fluorescence in green channel (H, * in J) showing regulatory elements in z ER81 _1.0kb reporter are not active in the thalamus.

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