An in vivo comparison of photoactivatable fluorescent proteins in an avian embryo model
Article first published online: 7 MAY 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Segmentation
Volume 236, Issue 6, pages 1583–1594, June 2007
How to Cite
Stark, D. A. and Kulesa, P. M. (2007), An in vivo comparison of photoactivatable fluorescent proteins in an avian embryo model. Dev. Dyn., 236: 1583–1594. doi: 10.1002/dvdy.21174
- Issue published online: 16 MAY 2007
- Article first published online: 7 MAY 2007
- Manuscript Accepted: 3 APR 2007
- Stowers Institute for Medical Research
The Supplementary Material referred to in this article can be found at http://www.interscience.wiley.com/jpages/1058-8388/suppmat
|jws-dvdy.21174.anim1.avi||2650K||Supplementary Movie S1: The photoactivation of Kaede A single cell labeled with Kaede is seen over time during the photoactivation with a 405nm laser. A line profile of the cell was taken to support the decrease of green intensity while the red intensity increased.|
|jws-dvdy.21174.anim2.AVI||1536K||Supplementary Movie S2: PSCFP2 photoactivated comparison of single-photon versus two-photon A 3D rendering of two photoactivated PSCFP2 cells where the top cell was photoactivated by 2-photon and the second cell was done with a 405nm laser. The left side of the movie represents how the 2-photon photoactivation is capable of targeting a single cell where a single-photon has an out of focus photoactivation effect. The right side demonstrates the density of the labeled neural tube cells and how it can present a challenge when trying to photoactivate single cells with a single-photon laser.|
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