Zenjiro Maruyama, Carolina A. Yoshida, and Tatsuya Furuichi contributed equally to this work.
Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency
Version of Record online: 11 MAY 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Ciona
Volume 236, Issue 7, pages 1876–1890, July 2007
How to Cite
Maruyama, Z., Yoshida, C. A., Furuichi, T., Amizuka, N., Ito, M., Fukuyama, R., Miyazaki, T., Kitaura, H., Nakamura, K., Fujita, T., Kanatani, N., Moriishi, T., Yamana, K., Liu, W., Kawaguchi, H., Nakamura, K. and Komori, T. (2007), Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency. Dev. Dyn., 236: 1876–1890. doi: 10.1002/dvdy.21187
- Issue online: 18 JUN 2007
- Version of Record online: 11 MAY 2007
- Manuscript Accepted: 10 APR 2007
- Ministry of Education, Culture, Sports, Science, and Technology, Japan
- Sumitomo Foundation
- bone metabolism
Runx2 is an essential transcription factor for osteoblast differentiation. However, the functions of Runx2 in postnatal bone development remain to be clarified. Introduction of dominant-negative (dn)-Runx2 did not inhibit Col1a1 and osteocalcin expression in mature osteoblastic cells. In transgenic mice that expressed dn-Runx2 in osteoblasts, the trabecular bone had increased mineralization, increased volume, and features of compact bone, and the expression of major bone matrix protein genes was relatively maintained. After ovariectomy, neither osteolysis nor bone formation was enhanced and bone was relatively conserved. In wild-type mice, Runx2 was strongly expressed in immature osteoblasts but downregulated during osteoblast maturation. These findings indicate that the maturity and turnover rate of bone are determined by the level of functional Runx2 and Runx2 is responsible for bone loss in estrogen deficiency, but that Runx2 is not essential for maintenance of the expression of major bone matrix protein genes in postnatal bone development and maintenance. Developmental Dynamics 236:1876–1890, 2007. © 2007 Wiley-Liss, Inc.