Analysis of Kupffer's vesicle in zebrafish embryos using a cave automated virtual environment
Version of Record online: 14 MAY 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Ciona
Volume 236, Issue 7, pages 1963–1969, July 2007
How to Cite
Kreiling, J. A., Prabhat, Williams, G. and Creton, R. (2007), Analysis of Kupffer's vesicle in zebrafish embryos using a cave automated virtual environment. Dev. Dyn., 236: 1963–1969. doi: 10.1002/dvdy.21191
- Issue online: 18 JUN 2007
- Version of Record online: 14 MAY 2007
- Manuscript Accepted: 16 APR 2007
- Nation Science Foundation. Grant Number: IOB-0421654
- Department of Energy. Grant Number: DEFG02-03ER25582
- Kupffer's vesicle;
- left/right asymmetry;
Previous studies show that cilia in Kupffer's vesicle (KV) generate a counterclockwise flow of fluid and provide convincing evidence that this flow regulates left/right asymmetry. We hypothesized that the distribution of cilia in KV leads to this directional flow. However, there are limitations in determining the localization of structures when viewing a three-dimensional (3-D) image on a 2-D computer screen. We analyzed the distribution of KV cilia in the Cave, an immersive virtual environment that displays stacks of confocal images in 3-D. We found 80% of the cilia are located on the dorsal surface and 20% were located on the ventral surface of the vesicle. We confirmed the ventral location of some cilia by electron microscopy. There is an asymmetrical distribution of cilia on the dorsal surface, with the anterior one third containing 50% and the posterior one third containing 20% of the cilia. This dorsal–anterior patch could explain the directionality of the flow, and could drive local differences in flow rate. Developmental Dynamics 236:1963–1969, 2007. © 2007 Wiley-Liss, Inc.