Drs. Pemberton and Li contributed equally to this work.
Patterns & Phenotypes
Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis
Version of Record online: 11 JUL 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 8, pages 2245–2257, August 2007
How to Cite
Pemberton, T. J., Li, F.-Y., Oka, S., Mendoza-Fandino, G. A., Hsu, Y.-H., Bringas, P., Chai, Y., Snead, M. L., Mehrian-Shai, R. and Patel, P. I. (2007), Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis. Dev. Dyn., 236: 2245–2257. doi: 10.1002/dvdy.21226
- Issue online: 25 JUL 2007
- Version of Record online: 11 JUL 2007
- Manuscript Accepted: 7 MAY 2007
- NIH-NIDCR. Grant Numbers: DE14102, DE06988
- National Center for Research Resources, National Institutes of Health. Grant Numbers: C06 (RR10600-01, CA62528-01, RR14514-01
- tooth development;
- gene expression;
- mRNA in situ hybridization
To identify genes heretofore undiscovered as critical players in the biogenesis of teeth, we have used microarray gene expression analysis of the developing mouse molar tooth (DMT) between postnatal day (P) 1 and P10 to identify genes differentially expressed when compared with 16 control tissues. Of the top 100 genes exhibiting increased expression in the DMT, 29 were found to have been previously associated with tooth development. Differential expression of the remaining 71 genes not previously associated with tooth development was confirmed by quantitative reverse transcription-polymerase chain reaction analysis. Further analysis of seven of the latter genes by mRNA in situ hybridization found that five were specific to the developing tooth in the craniofacial region (Rspo4, Papln, Amtn, Gja1, Maf). Of the remaining two, one was found to be more widely expressed (Sp7) and the other was found to be specific to the nasal serous gland, which is close to, but distinct from, the developing tooth (Vrm). Developmental Dynamics 236:2245–2257, 2007. © 2007 Wiley-Liss, Inc.