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In vivo quantum dot labeling of mammalian stem and progenitor cells

  1. Jonathan R. Slotkin1,2,†,
  2. Lina Chakrabarti3,†,
  3. Hai Ning Dai1,
  4. Rosalind S.E. Carney1,3,
  5. Tsutomu Hirata1,3,
  6. Barbara S. Bregman1,
  7. G. Ian Gallicano4,*,
  8. Joshua G. Corbin1,3,*,
  9. Tarik F. Haydar3,*

Article first published online: 11 JUL 2007

DOI: 10.1002/dvdy.21235

Issue

Developmental Dynamics

Developmental Dynamics

Special Issue: Special Focus on Stem Cells

Volume 236, Issue 12, pages 3393–3401, December 2007

Additional Information

How to Cite

Slotkin, J. R., Chakrabarti, L., Dai, H. N., Carney, R. S.E., Hirata, T., Bregman, B. S., Gallicano, G. I., Corbin, J. G. and Haydar, T. F. (2007), In vivo quantum dot labeling of mammalian stem and progenitor cells. Dev. Dyn., 236: 3393–3401. doi: 10.1002/dvdy.21235

Author Information

  1. 1

    Department of Neuroscience, Georgetown University School of Medicine, Washington, DC

  2. 2

    Department of Neurosurgery, The Brigham and Women's Hospital, The Children's Hospital, Harvard Medical School, Boston, Massachusetts

  3. 3

    Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center, Washington, DC

  4. 4

    Department of Cell Biology, Georgetown University School of Medicine, Washington, DC

  1. †

    Drs. Slotkin and Chakrabarti contributed equally to this work.

*Department of Cell Biology, Georgetown University School of Medicine, Washington, DC 20010

  1. †

    Drs. Slotkin and Chakrabarti contributed equally to this work.

Publication History

  1. Issue published online: 14 NOV 2007
  2. Article first published online: 11 JUL 2007
  3. Manuscript Accepted: 23 MAY 2007

Funded by

  • CNMC Board of Visitors
  • NINDS. Grant Numbers: NS051852, NS27054
  • National Alliance for Autism Research
  • Children's Research Institute, Washington DC
  • NIDA. Grant Number: DA020140
  • NICHD. Grant Number: HD007459
  • American Association of Neurological Surgeons/ Congress of Neurological Surgeons
  • Evident Technologies

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Keywords:

  • quantum dots;
  • stem cells;
  • development;
  • central nervous system

Abstract

Fluorescent semiconductor nanocrystal quantum dots (QDs) are a class of multifunctional inorganic fluorophores that hold great promise for clinical applications and biomedical research. Because no methods currently exist for directed QD-labeling of mammalian cells in the nervous system in vivo, we developed novel in utero electroporation and ultrasound-guided in vivo delivery techniques to efficiently and directly label neural stem and progenitor cells (NSPCs) of the developing mammalian central nervous system with QDs. Our initial safety and proof of concept studies of one and two-cell QD-labeled mouse embryos reveal that QDs are compatible with early mammalian embryonic development. Our in vivo experiments further show that in utero labeled NSPCs continue to develop in an apparent normal manner. These studies reveal that QDs can be effectively used to label mammalian NSPCs in vivo and will be useful for studies of in vivo fate mapping, cellular migration, and NSPC differentiation during mammalian development. Developmental Dynamics 236:3393–3401, 2007. © 2007 Wiley-Liss, Inc.

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