Drs. Slotkin and Chakrabarti contributed equally to this work.
Special Issue Techniques
In vivo quantum dot labeling of mammalian stem and progenitor cells
Article first published online: 11 JUL 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Stem Cells
Volume 236, Issue 12, pages 3393–3401, December 2007
How to Cite
Slotkin, J. R., Chakrabarti, L., Dai, H. N., Carney, R. S.E., Hirata, T., Bregman, B. S., Gallicano, G. I., Corbin, J. G. and Haydar, T. F. (2007), In vivo quantum dot labeling of mammalian stem and progenitor cells. Dev. Dyn., 236: 3393–3401. doi: 10.1002/dvdy.21235
- Issue published online: 14 NOV 2007
- Article first published online: 11 JUL 2007
- Manuscript Accepted: 23 MAY 2007
- CNMC Board of Visitors
- NINDS. Grant Numbers: NS051852, NS27054
- National Alliance for Autism Research
- Children's Research Institute, Washington DC
- NIDA. Grant Number: DA020140
- NICHD. Grant Number: HD007459
- American Association of Neurological Surgeons/ Congress of Neurological Surgeons
- Evident Technologies
- quantum dots;
- stem cells;
- central nervous system
Fluorescent semiconductor nanocrystal quantum dots (QDs) are a class of multifunctional inorganic fluorophores that hold great promise for clinical applications and biomedical research. Because no methods currently exist for directed QD-labeling of mammalian cells in the nervous system in vivo, we developed novel in utero electroporation and ultrasound-guided in vivo delivery techniques to efficiently and directly label neural stem and progenitor cells (NSPCs) of the developing mammalian central nervous system with QDs. Our initial safety and proof of concept studies of one and two-cell QD-labeled mouse embryos reveal that QDs are compatible with early mammalian embryonic development. Our in vivo experiments further show that in utero labeled NSPCs continue to develop in an apparent normal manner. These studies reveal that QDs can be effectively used to label mammalian NSPCs in vivo and will be useful for studies of in vivo fate mapping, cellular migration, and NSPC differentiation during mammalian development. Developmental Dynamics 236:3393–3401, 2007. © 2007 Wiley-Liss, Inc.