Patterns & Phenotypes
Expression profiles of the duplicated matrix metalloproteinase-9 genes suggest their different roles in apoptosis of larval intestinal epithelial cells during Xenopus laevis metamorphosis
Version of Record online: 25 JUL 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 8, pages 2338–2345, August 2007
How to Cite
Hasebe, T., Kajita, M., Fujimoto, K., Yaoita, Y. and Ishizuya-Oka, A. (2007), Expression profiles of the duplicated matrix metalloproteinase-9 genes suggest their different roles in apoptosis of larval intestinal epithelial cells during Xenopus laevis metamorphosis. Dev. Dyn., 236: 2338–2345. doi: 10.1002/dvdy.21252
- Issue online: 25 JUL 2007
- Version of Record online: 25 JUL 2007
- Manuscript Accepted: 5 JUN 2007
- JSPS Grants-in-Aid for Scientific Research (C). Grant Number: 17570051
- Ministry of Education, Science, and Culture of Japan
- matrix metalloproteinase-9 (MMP-9);
- Xenopus laevis;
- intestinal remodeling;
Matrix metalloproteinases (MMPs) play a pivotal role in development and/or pathogenesis through degrading extracellular matrix (ECM) components. We have previously shown that Xenopus MMP-9 gene is duplicated. To assess possible roles of MMP-9 and MMP-9TH in X. laevis intestinal remodeling, we here analyzed their expression profiles by in situ hybridization and show that their expression is transiently up-regulated during thyroid hormone-dependent metamorphosis. Of interest, MMP-9TH mRNA is strictly localized in the connective tissue and most highly expressed just beneath the larval epithelium that begins to undergo apoptosis. On the other hand, cells expressing MMP-9 mRNA become first detectable in the connective tissue and then, after the start of epithelial apoptosis, also in the larval epithelium. These results strongly suggest that MMP-9TH is responsible in the larval epithelial apoptosis through degrading ECM components in the basal lamina, whereas MMP-9 is involved in the removal of dying epithelial cells during amphibian intestinal remodeling. Developmental Dynamics 236:2338–2345, 2007. © 2007 Wiley-Liss, Inc.