Patterns & Phenotypes
Bone growth in zebrafish fins occurs via multiple pulses of cell proliferation
Article first published online: 5 AUG 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 9, pages 2668–2674, September 2007
How to Cite
Jain, I., Stroka, C., Yan, J., Huang, W.-M. and Iovine, M. K. (2007), Bone growth in zebrafish fins occurs via multiple pulses of cell proliferation. Dev. Dyn., 236: 2668–2674. doi: 10.1002/dvdy.21270
- Issue published online: 17 AUG 2007
- Article first published online: 5 AUG 2007
- Manuscript Accepted: 22 JUN 2007
- NIDCR. Grant Number: DE014863
- NIH-NCRR. Grant Number: P40 RR12546
- bone growth;
- segment length;
Fin length in the zebrafish is achieved by the distal addition of bony segments of the correct length. Genetic and molecular data provided evidence that segment growth uses a single pulse of growth, followed by a period of stasis. Examination of cell proliferation during segment growth was predicted to expose a graphical model consistent with a single burst of cell division (e.g., constant, parabolic, or exponential decay) during the lengthening of the distal-most segment. Cell proliferation was detected either by labeling animals with bromodeoxyuridine (during S-phase) or monitoring histone3-phosphate (mitosis). Results from both methods revealed that the number of proliferating cells fluctuates in apparent pulses as a segment grows (i.e., during the growth phase). Thus, rather than segment size being the result of a single burst of proliferation, it appears that segment growth is the result of several pulses of cell division that occur approximately every 60 microns (average segment length ∼ 250 microns). These results indicate that segment lengthening requires multiple pulses of cell proliferation. Developmental Dynamics 236:2668–2674, 2007. © 2007 Wiley-Liss, Inc.