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The Supplementary Material referred to in this article can be found at http://www.interscience.wiley.com/jpages/1058-8388/suppmat

FilenameFormatSizeDescription
jws-dvdy.21324.anim1.mov2256K Supplementary Movie 1 Apical protrusions of columnar epithelial cellsA movie showing different views of a 3D-rendered living wing imaginal disc expressing CD8-GFP in the dorsal compartment (green;ap-GAL4, UAS-CD8-GFP , Fig. 1B-D). Scale bar, 10 μm.
jws-dvdy.21324.anim2.mov4131K Supplementary Movies 2 and 3 Apical protrusions of columnar epithelial cells contact the squamous epithelium. Movies showing different views of a 3D-rendered living wing imaginal disc expressing CD8-GFP (green;act5c-GAL4, UAS-CD8-GFP , Fig. 1E-H') in clones of cells and stained with the lipophilic dye FM4-64 (red). The red channel, corresponding to FM4-64, is removed in Supplementary movie 2 to better show CD8-GFP-labeled apical protrusions. Scale bar, 10 μm.
jws-dvdy.21324.anim3.mov5122K Supplementary Movies 2 and 3 Apical protrusions of columnar epithelial cells contact the squamous epithelium. Movies showing different views of a 3D-rendered living wing imaginal disc expressing CD8-GFP (green;act5c-GAL4, UAS-CD8-GFP , Fig. 1E-H') in clones of cells and stained with the lipophilic dye FM4-64 (red). The red channel, corresponding to FM4-64, is removed in Supplementary movie 2 to better show CD8-GFP-labeled apical protrusions. Scale bar, 10 μm.
jws-dvdy.21324.anim4.mov10755K Supplementary Movie 4 Apical protrusions contain actinA movie showing different views of a 3D-rendered living wing imaginal disc expressing GFP-actin in the dorsal compartment (green;ap-GAL4, UAS-GFP-actin , Fig. 2C-E). Scale bar, 10 μm.
jws-dvdy.21324.anim5.mov21865K Supplementary Movies 5 and 6 A GFP-tagged form of the Dpp receptor Thickveins localizes to apical protrusions.Movies showing different views of a 3D-rendered living wing imaginal disc expressing Tkv-GFP in the dorsal compartment (green;ap-GAL4, UAS-tkv-GFP , Fig. 5C,C') and stained with the lipophilic dye FM4-64 (red). The red channel, corresponding to FM4-64, is removed in Supplementary movie 6. Scale bar, 10 μm.
jws-dvdy.21324.anim6.mov14906K Supplementary Movies 5 and 6 A GFP-tagged form of the Dpp receptor Thickveins localizes to apical protrusions.Movies showing different views of a 3D-rendered living wing imaginal disc expressing Tkv-GFP in the dorsal compartment (green;ap-GAL4, UAS-tkv-GFP , Fig. 5C,C') and stained with the lipophilic dye FM4-64 (red). The red channel, corresponding to FM4-64, is removed in Supplementary movie 6. Scale bar, 10 μm.
jws-dvdy.21324.fig1.tif2107K Supplementary Figure 1 Localization ofDrosophilaProminin-like-GFP in basolateral and basal sections of the wing disc columnar epithelium. A basolateral (A-A") and basal (B-B") section of the wing disc columnar epithelium expressing theDrosophilaProminin-like fusion protein in the dorsal compartment ( ap-GAL4, UAS-prominin-like-GFP ). Wing imaginal discs were stained for the basolateral membrane component Fasciclin III (FasIII, red), the adherens junction component DE-Cadherin (DE-Cad, blue) and Prominin-like-GFP (green). (A-A") No GFP immunoreactivity is detected in basolateral section of the wing disc columnar epithelium. (B-B") GFP immunoreactivity is detected in basal sections of the wing disc columnar epithelium in punctate structures. No GFP immunoreactivity is detected in the ventral compartment (lower part of the micrographs B-B"). Scale bar, 20 μm.
jws-dvdy.21324.fig2.tif1555K Supplementary Figure 2 Squamous epithelial cells project cell protrusions towards the columnar epithelium, as previously described (Cho et al., 2000; Gibson and Schubiger, 2000). (A, B, C) Different views of a 3D-rendering of a live wing imaginal disc stained with the lipophilic dye FM4-64. Squamous epithelial (s.e.) cells project cell protrusions (asterisks) towards the columnar epithelium (c.e.). Scale bar, 10 μm.

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