This article is a US Government work and, as such, is in the public domain in the United States of America.
Gonadotropin-releasing hormone-1 (GnRH-1) is involved in tooth maturation and biomineralization†
Article first published online: 18 OCT 2007
Published 2007 Wiley-Liss, Inc.
Volume 236, Issue 11, pages 2980–2992, November 2007
How to Cite
Tiong, J., Locastro, T. and Wray, S. (2007), Gonadotropin-releasing hormone-1 (GnRH-1) is involved in tooth maturation and biomineralization. Dev. Dyn., 236: 2980–2992. doi: 10.1002/dvdy.21332
- Issue published online: 18 OCT 2007
- Article first published online: 18 OCT 2007
- Manuscript Accepted: 9 AUG 2007
- Intramural Research Program of the NINDS, NIH
- GnRH-1 receptor;
- tooth development and biomineralization;
- GnRH-1-deficient mice
Gonadotropin releasing-hormone-1 (GnRH-1) is expressed in mouse incisors during development. In this report, we identify (1) cell type(s) that express GnRH-1 throughout tooth development, (2) the GnRH-1 receptor, and (3) the role of GnRH-1/GnRH-1 receptor signaling in tooth maturation. Results show that GnRH-1-positive cells in dental epithelium differentiate and populate multiple tooth structures including ameloblast and papillary layers that are involved in enamel formation and mineralization. The GnRH-1 receptor was present, and in vitro a GnRH-1 antagonist attenuated incisor GnRH-1 cell expression. In vivo, in mice lacking GnRH-1 (−/−), the incisors were discolored, longer, and more curved compared to wildtype. Elemental analysis of calcium, phosphorus, and iron revealed changes in −/− incisors consistent with GnRH-1 affecting movement of minerals into the dental matrix. In sum, in tooth development a signal transduction pathway exists for GnRH-1 via the GnRH-1 receptor and disruption of such signaling affects incisor growth and biomineralization. Developmental Dynamics 236:2980–2992, 2007. Published 2007 Wiley-Liss, Inc.