Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos
Article first published online: 18 OCT 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 237, Issue 1, pages 39–50, January 2008
How to Cite
Sasakura, Y., Konno, A., Mizuno, K., Satoh, N. and Inaba, K. (2008), Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos. Dev. Dyn., 237: 39–50. doi: 10.1002/dvdy.21333
- Issue published online: 19 DEC 2007
- Article first published online: 18 OCT 2007
- Manuscript Accepted: 23 AUG 2007
- MEXT, Japan. Grant Number: 18770193
- NS. Grant Number: 17207013
- NIG Cooperative Research Program. Grant Numbers: 2006-A72, 2007-B01
- enhancer trap;
Germline transgenesis with a Tc1/mariner superfamily Minos transposon was achieved in the ascidian Ciona intestinalis. Transgenic lines that express transposases in germ cells are very useful for remobilizing transposon copies. In the present study, we created transposase-expressing lines of Minos in Ciona. A Ciona gene encoding protamine (Ci-prm) is expressed in the testes and sperm. Transgenic lines expressing Minos transposase in the testes and sperm were created with a cis-element of Ci-prm, and used for enhancer detection. Double-transgenic animals between transposase lines and a transgenic line with an enhancer detection vector passed on several independent enhancer detection events to subsequent progeny. This technique allowed us to isolate transgenic lines that express GFP in restricted tissues. This system provides an easy and efficient method for large-scale enhancer detection in Ciona intestinalis. Developmental Dynamics 237:39–50, 2008. © 2007 Wiley-Liss, Inc.