Drs. Bashirullah and Lam contributed equally to this work.
Patterns & Phenotypes
dTrf2 is required for transcriptional and developmental responses to ecdysone during Drosophila metamorphosis
Version of Record online: 18 OCT 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 11, pages 3173–3179, November 2007
How to Cite
Bashirullah, A., Lam, G., Yin, V. P. and Thummel, C. S. (2007), dTrf2 is required for transcriptional and developmental responses to ecdysone during Drosophila metamorphosis. Dev. Dyn., 236: 3173–3179. doi: 10.1002/dvdy.21350
- Issue online: 18 OCT 2007
- Version of Record online: 18 OCT 2007
- Manuscript Accepted: 30 AUG 2007
- Howard Hughes Medical Institute
- steroid hormone;
The TATA box-binding protein (TBP) related factor 2 (TRF2) has been well characterized at a biochemical level and in cultured cells. Relatively little, however, is known about how TRF2 functions in specific biological pathways during development. Here, we show that Drosophila TRF2 (dTRF2) plays an essential role in responses to the steroid hormone ecdysone during the onset of metamorphosis. Hypomorphic dTrf2 mutations lead to developmental arrest during prepupal and early pupal stages with defects in major ecdysone-triggered biological responses, including puparium formation, anterior spiracle eversion, gas bubble translocation, adult head eversion, and larval salivary gland cell death. The transcription of key ecdysone-regulated target genes is delayed and reduced in dTrf2 mutants. dTrf2 appears to be required for the proper timing and levels of ecdysone-regulated gene expression required for entry into metamorphosis. Developmental Dynamics 236:3173–3179, 2007. © 2007 Wiley-Liss, Inc.