This article was accepted for inclusion in Developmental Dynamics236 #6—Special Focus on Segmentation.
Special Issue Patterns & Phenotypes
Gene expression patterns underlying proximal–distal skeletal segmentation in late-stage zebrafish, Danio rerio†
Article first published online: 18 OCT 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 236, Issue 11, pages 3111–3128, November 2007
How to Cite
Crotwell, P. L. and Mabee, P. M. (2007), Gene expression patterns underlying proximal–distal skeletal segmentation in late-stage zebrafish, Danio rerio. Dev. Dyn., 236: 3111–3128. doi: 10.1002/dvdy.21352
- Issue published online: 18 OCT 2007
- Article first published online: 18 OCT 2007
- Manuscript Accepted: 10 AUG 2007
- National Science Foundation/EPSCoR. Grant Number: EPS-0091948
- Sigma Xi, the Society of Integrative and Comparative Biology
- University of South Dakota
- State of South Dakota
- median fin;
- skeletal augmentation;
- gene expression;
Timing and pattern of expression of ten candidate segmentation genes or gene pairs were reviewed or examined in developing median fins of late-stage zebrafish, Danio rerio. We found a general correspondence in timing and pattern of expression between zebrafish fin radial segmentation and tetrapod joint development, suggesting that molecular mechanisms underlying radial segmentation have been conserved over 400 million years of evolution. Gene co-expression during segmentation (5.5–6.5 mm SL) is similar between tetrapods and zebrafish: bmp2b, bmp4, chordin, and gdf5 in interradial mesenchyme and ZS; bapx1, col2a1, noggin3, and sox9a in chondrocytes. Surprisingly, wnt9a is not expressed in the developing median fins, though wnt9b is detected. In contrast to all other candidate segmentation genes we examined, bapx1 is not expressed in the caudal fin, which does not segment. Together, these data suggest a scenario of gene interactions underlying radial segmentation based on the patterns and timing of candidate gene expression. Developmental Dynamics 236:3111–3128, 2007 © 2007 Wiley-Liss, Inc.