M. Bonds and J. Sands contributed equally to this work.
Patterns & Phenotypes
Genetic screen for regulators of ind expression identifies shrew as encoding a novel twisted gastrulation-like protein involved in Dpp signaling
Article first published online: 29 OCT 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Stem Cells
Volume 236, Issue 12, pages 3524–3531, December 2007
How to Cite
Bonds, M., Sands, J., Poulson, W., Harvey, C. and Von Ohlen, T. (2007), Genetic screen for regulators of ind expression identifies shrew as encoding a novel twisted gastrulation-like protein involved in Dpp signaling. Dev. Dyn., 236: 3524–3531. doi: 10.1002/dvdy.21360
- Issue published online: 14 NOV 2007
- Article first published online: 29 OCT 2007
- Manuscript Accepted: 15 SEP 2007
- National Center for Research Resources (NCRR). Grant Number: P20 RR016475
- Deficiency screen;
- dpp signaling;
Initiation and refinement of expression of the Ind homeodomain protein in the Drosophila embryo is coordinately regulated by global dorsoventral patterning pathways Dorsal, Egfr, and Dpp, and well as by Vnd, which positions the ventral boundary of Ind. Therefore, we set out to look for novel regulators of dorsoventral patterning by screening the Exelixis deficiency collection for modified expression of Ind. Indeed, we found deficiencies that remove components of the known signaling pathways had altered or lost ind expression. These findings included deficiencies that remove screw, dpp, and egfr as well as deficiencies that remove ind itself. In addition, we found several deficiencies that had altered or loss of ind expression. We also observed phenotypes suggestive of dorsoventral patterning defects such as twisting during gastrulation, and defects associated with loss of dorsal specification. These include a pair of overlapping deficiencies that produced ventralized embryos. We find that transheterozygotes of these two deficiencies are also ventralized. There are seven genes common to both deficiencies, including CG11582, which encodes a twisted gastrulation-like protein. These two deficiencies are also allelic with shrew mutations. Here, we present data supporting the conclusion that CG11582 is the gene affected in shrew mutants. Developmental Dynamics 236:3524–3531, 2007. © 2007 Wiley-Liss, Inc.