GAD isoforms exhibit distinct spatiotemporal expression patterns in the developing mouse lens: Correlation with Dlx2 and Dlx5

Authors

  • Andrea Kwakowsky,

    1. Department of Gene Technology and Developmental Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
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    • A. Kwakowsky and M. Schwirtlich contributed equally to this work.

  • Marija Schwirtlich,

    1. Department of Gene Technology and Developmental Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
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    • A. Kwakowsky and M. Schwirtlich contributed equally to this work.

  • Qi Zhang,

    1. Department of Human Anatomy and Cell Science, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
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  • David D. Eisenstat,

    1. Department of Human Anatomy and Cell Science, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
    2. Department of Pediatrics and Child Health, and Ophthalmology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada
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  • Ferenc Erdélyi,

    1. Department of Gene Technology and Developmental Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
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  • Mária Baranyi,

    1. Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
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  • Zoya D. Katarova,

    1. Department of Gene Technology and Developmental Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
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    • Drs. Katarova and Szabo share senior authorship.

  • Gábor Szabó

    Corresponding author
    1. Department of Gene Technology and Developmental Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
    • Institute of Experimental Medicine, Hungarian Academy of Sciences, 1450-Budapest, PO Box 67, Hungary
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    • Drs. Katarova and Szabo share senior authorship.


Abstract

Gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter of the adult nervous system and its biosynthetic enzyme glutamic acid decarboxylase (GAD) are abundantly expressed in the embryonic nervous system and are involved in the modulation of cell proliferation, migration, and differentiation. Here we describe for the first time the expression of GABA and embryonic and adult GAD isoforms in the developing mouse lens. We show that the GAD isoforms are sequentially induced with specific spatiotemporal profiles: GAD65 and embryonic GAD isoforms prevail in primary fibers, while GAD67 is the predominant GAD expressed in the postnatal secondary fibers. This pattern correlates well with the expression of Dlx2 and Dlx5, known as upstream regulators of GAD. GABA and GAD are most abundant at the tips of elongating fibers and are absent from organelle-free cells, suggesting their involvement is primarily in shaping of the cytoskeleton during fiber elongation stages. Developmental Dynamics 236:3532–3544, 2007. © 2007 Wiley-Liss, Inc.

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