Regulation and function of Dbx genes in the zebrafish spinal cord
Article first published online: 9 NOV 2007
Copyright © 2007 Wiley-Liss, Inc.
Special Issue: Special Focus on Stem Cells
Volume 236, Issue 12, pages 3472–3483, December 2007
How to Cite
Gribble, S. L., Nikolaus, O. B. and Dorsky, R. I. (2007), Regulation and function of Dbx genes in the zebrafish spinal cord. Dev. Dyn., 236: 3472–3483. doi: 10.1002/dvdy.21367
- Issue published online: 14 NOV 2007
- Article first published online: 9 NOV 2007
- Manuscript Accepted: 29 SEP 2007
- National Institutes of Health. Grant Numbers: RO1NS053897, T32 GM07464
- spinal cord;
- retinoic acid
Dbx homeodomain proteins are important for spinal cord dorsal/ventral patterning and the production of multiple spinal cord cell types. We have examined the regulation and function of Dbx genes in the zebrafish. We report that Hedgehog signaling is not required for the induction or maintenance of these genes; in the absence of Hedgehog signaling, dbx1a/1b/2 are expanded ventrally with concomitant increases in postmitotic neurons that differentiate from this domain. Also, we find that retinoic acid signaling is not required for the induction of Dbx expression. Furthermore, we are the first to report that knockdown of Dbx1 function causes a dorsal expansion of nkx6.2, which is thought to be the cross-repressive partner of Dbx1 in mouse. Our data confirm that the dbx1a/1b/2 domain in zebrafish spinal cord development behaves similarly to amniotes, while extending knowledge of Dbx1 function in spinal cord patterning. Developmental Dynamics 236:3472–3483, 2007. © 2007 Wiley-Liss, Inc.